Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana.

Wojtas M; Swiezewski S; Sarnowski TJ; Plochocka D; Chelstowska A; Tolmachova T; Swiezewska E

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Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana.

机译：拟南芥Rab Escort蛋白（REP）的克隆和鉴定。

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Intracellular vesicular trafficking is regulated by Rab proteins, small GTPases that require posttranslational geranylgeranylation for biological activity. This covalent modification is catalyzed by Rab geranylgeranyl transferase (RabGGTase) and proceeds only in the presence of accessory Rab Escort Protein (REP). In this communication, we report the cloning and characterization of REP gene of Arabidopsis thaliana. Highest expression of REP mRNA was detected in leaves and flowers in contrast to stems and roots. AtREP is recognized by anti-rat REP1 serum. Interaction of AtREP with the protein substrate is presented, as well as a structural model obtained through homology modeling, based on the known structure of rat REP1.

机译：细胞内囊泡运输受Rab蛋白（小型GTPases）调节，Rab蛋白需要翻译后的geranylgeranyylation具有生物学活性。这种共价修饰被Rab香叶基香叶基转移酶（RabGGTase）催化，并且仅在存在辅助性Rab Escort蛋白（REP）的情况下进行。在本交流中，我们报道了拟南芥REP基因的克隆和鉴定。与茎和根相比，在叶片和花朵中检测到REP mRNA的最高表达。 AtREP被抗大鼠REP1血清识别。提出了AtREP与蛋白质底物的相互作用，以及基于大鼠REP1的已知结构，通过同源性建模获得的结构模型。

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《Cell biology international.》 |2007年第3期|共6页
作者
Wojtas M; Swiezewski S; Sarnowski TJ; Plochocka D; Chelstowska A; Tolmachova T; Swiezewska E;
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正文语种 eng
中图分类细胞生物学;
关键词
Protein measurement; Arabidopsis thaliana; Cloning; REP1; Proteins; 蛋白质类;

机译：Protein measurement;Arabidopsis thaliana;Cloning;REP1;Proteins;蛋白质类;

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1. Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana. [J] . Wojtas M, Swiezewski S, Sarnowski TJ, Cell biology international. . 2007,第3期

机译：拟南芥Rab Escort蛋白（REP）的克隆和鉴定。
2. Caenorhabditis elegans Rab escort protein (REP-1) differently regulates each Rab protein function and localization in a tissue-dependent manner [J] . Tanaka Daisuke, Kameyama Kimihiko, Okamoto Harumasa, Genes to cells : . 2008,第11期

机译：秀丽隐杆线虫Rab伴游蛋白（REP-1）以组织依赖性方式不同地调节每个Rab蛋白的功能和定位
3. A Specific Feature of the Angiosperm Rab Escort Protein (REP) and Evolution of the REP/GDI Superfamily. [J] . Hala M, Elias M, Zarsky V Journal of Molecular Biology . 2005,第5期

机译：被子植物Rab护送蛋白（REP）的特定功能和REP / GDI超家族的进化。
4. Role of the PYL8/RCAR3 in abscisic acid (ABA)-mediated signalling in Arabidopsis thaliana. [C] . Chhaya B Patole, Jim Dunwell, Rainer Cramer ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：Pyl8 / Rcar3在拟南芥中的脱盐酸（ABA）介导的信号传导中的作用。
5. Purification,cloning,and characterization of a putative target protein for Rab3A small GTP-binding protein利用統計を見る [D] . Shirataki Hiromichi 1995

机译：利用统计を见る对Rab3A小GTP结合蛋白的推定靶蛋白进行纯化，克隆和鉴定
6. Loss-of-Function Mutations in Rab Escort Protein 1 (REP-1) Affect Intracellular Transport in Fibroblasts and Monocytes of Choroideremia Patients [O] . Natalia V. Strunnikova, Jennifer Barb, Yuri V. Sergeev, 2009

机译：Rab护送蛋白1（REP-1）的功能丧失突变影响脉络膜血友病患者成纤维细胞和单核细胞的细胞内转运
7. Membrane Targeting of a Rab GTPase That Fails to Associate with Rab Escort Protein (REP) or Guanine Nucleotide Dissociation Inhibitor (GDI)* [O] . Jean H. Overmeyer, Amy L. Wilson, William A. Maltese 2001

机译：RAB GTP酶的膜靶向，其未能与RAB护送蛋白（REP）或鸟嘌呤核苷酸解离抑制剂（GDI）*缔合

Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana.

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