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首页> 外文期刊>Cell biology international. >ECM remodelling components regulated during jaw periosteal cell osteogenesis.
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ECM remodelling components regulated during jaw periosteal cell osteogenesis.

机译：ECM重塑组件在颌骨骨膜细胞成骨过程中受到调节。

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Human JPCs (jaw periosteal cells) are a promising source for the engineering of cell-based osteoinductive grafts in oral surgery. For this purpose, cell characteristics of this stem cell source should be elucidated in detail. Analysis of gene expression profiles may help us to evaluate key factors and cellular targets of JPC osteogenesis. Because little is known about the interplay of osteogenic-related components, we analysed the expression of different collagen types reflecting important players for extracellular matrix assembly and of TIMPs (tissue inhibitors of metalloproteinases) responsible for the inhibition of matrix degradation. Gene expression analyses using microarrays and quantitative RT-PCR (reverse transcription-PCR) during JPC osteogenesis revealed the induction of several collagen types' expression (VII, VIII, XI and XII), and some of them (types I, VIII and XI) seemed to be susceptible to BMP-2 (bone morphogenetic protein-2) that is known to be a potent osteogenic inducer of periosteal cells. Among the TIMPs, only TIMP-4 and RECK (reversion-inducing cysteine-rich protein with Kazal motifs) expressions were strongly up-regulated during JPC osteogenesis. Proteome profiler analysis of supernatants from untreated and differentiated JPCs confirmed the gene expression data in terms of TIMP expression. In summary, we identified new collagen types and TIMPs that seem to play important roles during the osteogenesis of jaw periosteal progenitor cells.
机译：人类JPC（颌骨骨膜细胞）是在口腔手术中工程化基于细胞的骨诱导性移植物的有前途的来源。为此，应详细阐明该干细胞来源的细胞特征。基因表达谱的分析可能有助于我们评估JPC成骨的关键因素和细胞靶标。由于对成骨相关成分之间的相互作用知之甚少，我们分析了反映不同类型胶原蛋白的表达，这些胶原蛋白反映了细胞外基质组装的重要参与者以及负责抑制基质降解的TIMP（金属蛋白酶的组织抑制剂）。在JPC成骨过程中使用微阵列和定量RT-PCR（逆转录PCR）进行基因表达分析，揭示了几种胶原蛋白类型（VII，VIII，XI和XII）的表达，其中一些（I，VIII和XI型）被诱导似乎对BMP-2（骨形态发生蛋白2）敏感，BMP-2是骨膜细胞的有效成骨诱导剂。在TIMP中，在JPC成骨过程中，只有TIMP-4和RECK（具有Kazal基序的诱导还原的富含半胱氨酸的蛋白质）表达强烈上调。来自未经处理和分化的JPC的上清液的蛋白质组分析仪分析证实了TIMP表达方面的基因表达数据。总之，我们确定了新的胶原蛋白类型和TIMPs，它们在颌骨骨膜祖细胞的成骨过程中似乎起着重要作用。

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