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首页> 外文期刊>Life sciences >Effect of 1,25(OH)2D3 on expression of estrogen receptor-alpha mRNA on rat osteosarcoma cell line (ROS 17/2.8).
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Effect of 1,25(OH)2D3 on expression of estrogen receptor-alpha mRNA on rat osteosarcoma cell line (ROS 17/2.8).

机译：1,25（OH）2D3对大鼠骨肉瘤细胞系（ROS 17 / 2.8）雌激素受体αmRNA表达的影响。

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摘要

In order to investigate the regulatory mechanisms of estrogen receptors (ER) in bone cells, changes in ER-alpha mRNA levels of rat osteosarcoma cell line (ROS 17/2.8) before and after exposure to 1,25(OH)2D3 and 17-beta estradiol respectively were measured by quantitative polymerase chain reaction using an internal standard. ER mRNA levels in the ROS 17/2.8 cultured with the medium alone had 5.029 +/- 1.623 mol/g total RNA x 10(-13) and were not statistically different from those cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-12) M and less. ER mRNA levels in the ROS 17/2.8 cell line showed a small but a significant increase as a result of stimulation by 1,25(OH)2D3 at concentrations of 10(-10) and 10(-11) M. However, ER mRNA levels in ROS 17/2.8 cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-9) M were not statistically different from those of the control. On the other hand, the expression of ER in ROS 17/2.8 cells cultured for 3 hours with various doses of 1,25(OH)2D3 showed, by immunoblotting methods, a significant increase at the dose of 10(-10) M in the expression of ER. Although a physiological significance is obscure, these observations suggest that 1,25(OH)2D3 plays a part in the expression of ER in ROS 17/2.8. No significant changes were seen in the expression of ER mRNA and the synthesis of ER as a result of stimulation by the estradiol.
机译：为了研究骨细胞中雌激素受体（ER）的调节机制，在暴露于1,25（OH）2D3和17-之前和之后，大鼠骨肉瘤细胞系（ROS 17 / 2.8）ER-αmRNA水平的变化使用内标通过定量聚合酶链反应分别测定β-雌二醇。仅用培养基培养的ROS 17 / 2.8中的ER mRNA水平具有5.029 +/- 1.623 mol / g总RNA x 10（-13），与在1,25（OH）2D3存在下培养的ER mRNA水平无统计学差异在10（-12）M和更低的浓度下。在浓度为10（-10）和10（-11）M的条件下，通过1,25（OH）2D3的刺激，ROS 17 / 2.8细胞系中的ER mRNA水平显示出少量但显着的增加。但是，ER在浓度为10（-9）M的1,25（OH）2D3存在下培养的ROS 17 / 2.8中的mRNA水平与对照组无统计学差异。另一方面，通过免疫印迹法，在不同剂量的1,25（OH）2D3培养3小时的ROS 17 / 2.8细胞中ER的表达表明，在10（-10）M剂量下，ER的表达显着增加。 ER的表达。尽管其生理意义尚不清楚，但这些观察结果表明，1,25（OH）2D3在ROS 17 / 2.8中的ER表达中起作用。由于雌二醇的刺激，ER mRNA的表达和ER的合成均未见明显变化。

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