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Measurements of 1,2-diacylglycerol and ceramide in hearts subjected to ischemic preconditioning.

机译：接受缺血预处理的心脏中1，2-二酰基甘油和神经酰胺的测定。

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摘要

An accumulation of recent evidence suggests that the mechanism in ischemic preconditioning (IPC) may involve the activation of protein kinase C (PKC) regulatory pathway. In this study, we examined whether the content of 1,2-diacylglycerol (1,2-DAG) and ceramide, which are intracellular second messengers regulating PKC activity, change during IPC in isolated perfused rat hearts, and whether the observed change in 1,2-DAG is accompanied with alteration in its fatty acid composition. Hearts subjected to IPC, consisting of 5-min transient global ischemia followed by 5-min reperfusion, presented a significant functional recovery during subsequent 40-min reperfusion following 40-min global ischemia compared with non-preconditioned hearts. An increase in 1,2-DAG content was observed in hearts subjected to 5-min transient ischemia compared with non-ischemic control hearts, however this was not seen in hearts harvested after 5-min reperfusion following 5-min ischemia. While fatty acid composition in 1,2-DAG was virtually unchanged in hearts subjected to 5-min ischemia, saturated 1,2-DAG decreased and monounsaturated/polyunsaturated 1,2-DAG increased in hearts reperfused for 5-min following 5-min ischemia compared with the non-ischemic control hearts. Ceramide mass did not change significantly, suggesting that the contribution of ceramide may be small in IPC. These data are in concert with the hypothesis that 1,2-DAG is a second messenger in IPC and the changes in fatty acid composition of 1,2-DAG may add new insight concerning signal transduction pathway in IPC.
机译：最新证据的积累表明，缺血预处理（IPC）中的机制可能涉及蛋白激酶C（PKC）调节途径的激活。在这项研究中，我们检查了1,2-二甘油甘油（1,2-DAG）和神经酰胺的含量（它们是调节PKC活性的细胞内第二信使），在离体灌注大鼠心脏的IPC过程中是否发生变化，以及观察到的变化是否在1 ，2-DAG伴随着其脂肪酸组成的改变。与未预处理的心脏相比，接受IPC的心脏（包括5分钟的短暂性整体缺血，然后进行5分钟的再灌注）在随后的40分钟的整体缺血后的40分钟再灌注中表现出明显的功能恢复。与非缺血性对照心脏相比，在经历5分钟短暂性缺血的心脏中观察到1,2-DAG含量增加，但是在缺血5分钟后5分钟再灌注后收获的心脏中未观察到这种现象。在5分钟缺血的心脏中，1,2-DAG中的脂肪酸组成实际上没有变化，但在5分钟后再灌注5分钟的心脏中，饱和1,2-DAG减少，单不饱和/多不饱和1,2-DAG增加缺血与非缺血对照组相比。神经酰胺的质量没有明显变化，表明在IPC中神经酰胺的贡献可能很小。这些数据符合以下假设：1,2-DAG是IPC中的第二个信使，1,2-DAG脂肪酸组成的变化可能会增加有关IPC中信号转导途径的新见解。

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