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首页> 外文期刊>Life sciences >First demonstration of lactoribonuclease, a ribonuclease from bovine milk with similarity to bovine pancreatic ribonuclease
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First demonstration of lactoribonuclease, a ribonuclease from bovine milk with similarity to bovine pancreatic ribonuclease

机译：乳核糖核酸酶的首次展示，这是一种与牛胰腺核糖核酸酶相似的牛乳核糖核酸酶

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The isolation of a ribonuclease designated lactoribonuclease, with a molecular weight and an N-terminal amino acid sequence identical to those of bovine pancreatic ribonuclease, was first reported from bovine milk. After removal of globulin from acid whey by precipitation with 1.8 M (NH4)(2)SO4, (NH4)(2)SO4 was added to attain a concentration of 3.6M. Adsorption on the ion exchanger CM-Sepharose and subsequently on Mono S by fast protein liquid chromatography yielded pure lactoribonuclease. The enzyme, like pancreatic ribonuclease, was most active at pH 7.5 with yeast transfer RNA (tRNA) as substrate. Lactoribonuclease and pancreatic ribonuclease showed a strong preference for poly(C) over poly(U). However, pancreatic ribonuclease did so with a higher specific activity, suggesting that the two ribonucleases are not identical. No inhibitory effect was shown by either lactoribonuclease or pancreatic ribonuclease toward poly (A) and poly (G). The effect of lactoribonuclease and pancreatic ribonuclease on tRNA increased with the concentration of tRNA. Lactoribonuclease inhibited cell-free translation in a rabbit reticulocyte lysate system with an IC50 of 3.5 nM while the corresponding IC50 for pancreatic ribonuclease was 0.09 nM. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 15]
机译：首先从牛乳中报道了分离的命名为乳核糖核酸酶的核糖核酸酶，其分子量和N端氨基酸序列与牛胰核糖核酸酶的分子量和N端氨基酸序列相同。通过用1.8 M（NH4）（2）SO4沉淀从酸乳清中除去球蛋白后，添加（NH4）（2）SO4以达到3.6M的浓度。快速蛋白质液相色谱在离子交换剂CM-琼脂糖上吸附，然后在Mono S上吸附，得到纯的乳核糖核酸酶。该酶像胰核糖核酸酶一样，在pH 7.5下以酵母转移RNA（tRNA）为底物最活跃。乳酸核糖核酸酶和胰核糖核酸酶显示出对poly（C）的强烈偏爱，而不是poly（U）。但是，胰核糖核酸酶具有更高的比活性，这表明两个核糖核酸酶是不同的。乳核糖核酸酶或胰核糖核酸酶对聚（A）和聚（G）均未显示抑制作用。乳核糖核酸酶和胰核糖核酸酶对tRNA的影响随tRNA浓度的增加而增加。乳核糖核酸酶抑制兔网织红细胞裂解液系统中的无细胞翻译，IC50为3.5 nM，而胰核糖核酸酶的相应IC50为0.09 nM。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：15]

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