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Stimulation of sub-sonic vibration promotes the differentiation of adipose tissue-derived mesenchymal stem cells into neural cells

机译：亚音速振动的刺激促进了脂肪组织来源的间充质干细胞向神经细胞的分化

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Aims: Adipose tissue-derived stem cells (AT-MSCs) have been proposed as a new source for nervous tissue damage due to their capacity of neural differentiation potential including neurons, oligodendrocytes and astrocytes. Recently, many studies have demonstrated that sub-sonic vibration (SSV) is an effective cell differentiation method but there have been no studies on the effect of SSV about AT-MSC differentiation into neural-like cells in vitro. Therefore, we examined the effect of SSV on AT-MSCs to investigate the differentiation potential of neural-like cells. Main methods: We assessed the changes in AT-MSCs by SSV during 4 days at 10, 20, 30 and 40 Hz (1.0 V). After stimulation, they were analyzed by RT-PCR, Western blot and immunohistological analysis using neural cell type-specific genes and antibodies. Further, to confirm the neural differentiation, we investigated adipogenic genes for RT-PCR analysis. For a mechanism study, we analyzed activation levels in time course of ERK phosphorylation after SSV. Key findings: After 4-day SSV exposure, we observed morphological changes of AT-MSCs. Further, SSV induced gene/protein levels of neural markers while inhibiting adipogenesis and they were mainly upregulated at 30 Hz. In addition, phosphorylated ERK level was increased in a time-dependent manner upon 30 Hz SSV for 6 h. Significance: These results demonstrated that SSV affects AT-MSCs differentiation potential and 30 Hz SSV affected neural differentiation on AT-MSCs.
机译：目的：由于脂肪组织的干细胞具有神经分化潜能，包括神经元，少突胶质细胞和星形胶质细胞，因此已提出将其作为神经组织损伤的新来源。近年来，许多研究表明亚音速振动（SSV）是一种有效的细胞分化方法，但还没有关于SSV的体外AT-MSC分化为神经样细胞的效果的研究。因此，我们研究了SSV对AT-MSC的影响，以研究神经样细胞的分化潜能。主要方法：我们评估了SSV在10、20、30和40 Hz（1.0 V）下4天中AT-MSC的变化。刺激后，通过RT-PCR，蛋白质印迹和使用神经细胞类型特异性基因和抗体的免疫组织学分析对它们进行分析。此外，为了确认神经分化，我们研究了成脂基因，用于RT-PCR分析。为了进行机理研究，我们分析了SSV后ERK磷酸化过程中的激活水平。主要发现：SSV暴露4天后，我们观察到AT-MSC的形态变化。此外，SSV诱导神经标记的基因/蛋白质水平，同时抑制脂肪形成，并且它们主要在30 Hz上调。此外，在30 Hz SSV下持续6 h，磷酸化ERK水平以时间依赖性方式增加。意义：这些结果表明SSV影响AT-MSC的分化潜能，而30 Hz SSV影响AT-MSC的神经分化。

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