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首页> 外文期刊>Life sciences >Effects of interferon-alpha on cloned opioid receptors expressed in Xenopus oocytes.
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Effects of interferon-alpha on cloned opioid receptors expressed in Xenopus oocytes.

机译：干扰素-α对非洲爪蟾卵母细胞中表达的克隆阿片受体的影响。

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Interferon-alpha (IFNalpha) affects the opioid system. However, the direct action of IFNalpha on cloned opioid receptors remains unknown. Taking advantage of the functional coupling of cloned opioid receptors to G protein-activated inwardly rectifying K(+) (GIRK) channels in a Xenopus oocyte expression system, we investigated the effects of recombinant IFNalpha on cloned mu-, delta- and kappa-opioid receptors. In oocytes co-injected with mRNAs for either the delta- or kappa-opioid receptor and for GIRK channel subunits, IFNalpha at high concentrations induced small GIRK currents that were abolished by naloxone, an opioid-receptor antagonist, compared with the control responses to each selective opioid agonist. Additionally, IFNalpha induced no significant current response in oocytes injected with mRNA(s) for either opioid receptor alone or GIRK channels. In oocytes expressing the mu-opioid receptor and GIRK channels, IFNalpha had little or no effect. Moreover, in oocytes expressing each opioid receptorand GIRK channels, GIRK current responses to each selective opioid agonist were not affected by the presence of IFNalpha, indicating no significant antagonism of IFNalpha toward the opioid receptors. Furthermore, IFNalpha had little or no effect on the mu/delta-, delta/kappa- or mu/kappa-opioid receptors expressed together with GIRK channels in oocytes. Our results suggest that IFNalpha weakly activates the delta and kappa-opioid receptors. The direct activation of the delta- and kappa-opioid receptors by IFNalpha may partly contribute to some of the IFNalpha effects under its high-dose medication.
机译：干扰素-α（IFNalpha）影响阿片类药物系统。但是，IFNα对克隆的阿片受体的直接作用仍然未知。利用克隆的阿片受体功能耦合到非洲爪蟾卵母细胞表达系统中的G蛋白激活的内向整流K（+）（GIRK）通道，我们研究了重组IFNalpha对克隆的mu，delta和kappa类阿片的影响受体。与δ或κ阿片受体和GIRK通道亚基的mRNA共同注射的卵母细胞中，高浓度的IFNα诱导了小GIRK电流，与阿片受体拮抗剂纳洛酮相比，IFNα消除了该电流，相比之下，对每个阿片受体的对照反应选择性阿片类激动剂。另外，IFNα在注射了单独的阿片受体或GIRK通道的mRNA的卵母细胞中没有诱导明显的电流反应。在表达mu阿片受体和GIRK通道的卵母细胞中，IFNalpha几乎没有作用。而且，在表达每个阿片受体和GIRK通道的卵母细胞中，对每个选择性阿片激动剂的GIRK电流响应不受IFNα的存在的影响，表明IFNα对阿片受体没有明显的拮抗作用。此外，IFNα对卵母细胞中与GIRK通道一起表达的μ/δ，δ/κ或μ/κ阿片受体几乎没有影响。我们的结果表明，IFNα弱激活了δ和κ阿片受体。在大剂量药物作用下，IFNα直接激活δ和κ阿片受体可能部分地导致了某些IFNα效应。

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