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首页> 外文期刊>Life sciences >Palladium and platinum ions interfere with the measurement of erythrocyte vesiculation by inhibiting the acetylcholinesterase activity of the released spectrin-depleted microvesicles.
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Palladium and platinum ions interfere with the measurement of erythrocyte vesiculation by inhibiting the acetylcholinesterase activity of the released spectrin-depleted microvesicles.

机译：钯和铂离子通过抑制释放的血红蛋白耗尽的微囊泡的乙酰胆碱酯酶活性来干扰红细胞囊泡的测量。

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摘要

Palladium (Pd(2+)) and platinum (Pt(2+)) ions were found to inhibit erythrocyte membrane-bound acetylcholinesterase (AChE) with Ki values of 6.0 and 6.5 microg/ml, respectively. Lineweaver-Burke plots revealed that the inhibition of erythrocyte AChE by both metal ions was competitive in nature. Binding studies using alkaline phosphatase as a reporting enzyme confirmed that both metal ions indeed did bind to the enzyme molecules. In the process of red cell vesiculation, membrane-bound AChE is shed along with vesicles. The measurement of AChE activities in the medium containing vesiculated RBC could potentially be served as an index of vesiculation. Inhibition of AChE activities by both metal ions can thus constitute a potential source of error in vesiculation measurement. To illustrate these effects, a simulated vesiculation system, using green tea polyphenol in the presence (25 microg/ml) or absence of Pd(2+) ion was simultaneously examined by the electronmicrography and the AChE method. We observed vesiculation under the experimental condition in Pd(2+)-free controls that was associated with a time-dependent increase in AChE activity were barely detected in the Pd(2+)-spiked specimen because of the masking effect exerted by the metal ions themselves.
机译：发现钯（Pd（2+））和铂（Pt（2+））离子分别抑制Ki值为6.0和6.5 microg / ml的红细胞膜结合的乙酰胆碱酯酶（AChE）。 Lineweaver-Burke图显示，两种金属离子对红细胞AChE的抑制作用本质上都是竞争性的。使用碱性磷酸酶作为报告酶的结合研究证实，两种金属离子确实确实结合了酶分子。在红细胞囊泡化过程中，膜结合的AChE与囊泡一起脱落。含有泡状红细胞的培养基中AChE活性的测量可能会成为泡状指数。因此，两种金属离子对AChE活性的抑制都可能构成囊泡测量误差的潜在来源。为了说明这些效果，通过电子显微术和AChE方法同时检查了使用绿茶多酚存在（25微克/毫升）或不存在Pd（2+）离子的模拟泡囊系统。我们观察到在Pd（2+）掺入的标本中几乎没有检测到在无Pd（2+）的对照条件下的囊泡化，与AChE活性的时间依赖性增加相关，这是由于金属的掩盖作用离子本身。

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