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首页> 外文期刊>Life sciences >Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.
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Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.

机译：蛋白质组学对大鼠大脑中葡萄籽提取物作用的分析：对精神活性化合物作用的研究的技术和生物学意义。

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Grape seed extract (GSE) is a commonly available dietary supplement taken for the anti-oxidant activity that's attributed to its proanthocyanidin (oligomers of monomeric polyphenols) content. Similar polyphenol-enriched preparations from blueberries and soy have shown protection against ovariectomy-induced or age-related cognitive deficits, suggesting that the molecular changes induced by these polyphenol preparations correlated with behavioral benefit. We hypothesized that ingestion of polyphenol-enriched preparations such as GSE would be manifested as protein changes that would be consistent with neuroprotection. Proteomics technology, namely 2D gel electrophoresis and mass spectrometry, identified quantitative changes in specific proteins induced in adult rat brain following ingestion of a powdered preparation of GSE. As recently reported [Deshane, J., Chaves, L., Sarikonda, K.V., Isbell, S., Wilson, L., Kirk, M., Grubbs, C., Barnes, S., Meleth, S. and Kim, H., 2004. Proteomics analysis of rat brainprotein modulations by grape seed extract. Journal of Agricultural and Food Chemistry 52, 7872-7883.], the direction of change for the majority of the affected proteins was opposite to the direction the proteins were changed in either Alzheimer disease or in transgenic mouse models of dementia. A conservative conclusion is that GSE has neuroprotective activity, by affecting specific proteins in particular ways. In this chapter, elements of proteomics-type analysis are discussed that demonstrate the power of the technology to enable discovery of proteins involved in the response of the brain to a stimulus whether it be a dietary supplement, or a psychoactive drug. The fact that GSE affects proteins implicated in cognitive disorders suggests moreover that GSE may have impact on the actions of psychoactive drugs by maintaining an overall viability of the nervous system.
机译：葡萄籽提取物（GSE）是一种常用的膳食补充剂，由于其原花青素（单体多酚的低聚物）含量而具有抗氧化活性。蓝莓和大豆中类似的富含多酚的制剂已显示出对卵巢切除术引起的或年龄相关的认知缺陷的保护作用，表明这些多酚制剂诱导的分子变化与行为益处相关。我们假设摄入富含多酚的制剂（例如GSE）将表现为蛋白质变化，这与神经保护作用一致。蛋白质组学技术，即2D凝胶电泳和质谱法，确定了摄入GSE粉末制剂后在成年大鼠脑中诱导的特定蛋白质的定量变化。根据最近的报道[Deshane，J.，Chaves，L.，Sarikonda，KV，Isbell，S.，Wilson，L.，Kirk，M.，Grubbs，C.，Barnes，S.，Meleth，S.和Kim， H.，2004.葡萄籽提取物对大鼠脑蛋白调节的蛋白质组学分析。农业和食品化学学报52，7872-7883。]，大多数受影响蛋白质的变化方向与阿尔茨海默氏病或​​痴呆的转基因小鼠模型中蛋白质变化的方向相反。保守的结论是，GSE通过以特定方式影响特定蛋白质而具有神经保护活性。在本章中，将讨论蛋白质组学类型分析的要素，以证明该技术的强大功能，使人们能够发现与大脑对食物的刺激或精神活性药物的刺激有关的蛋白质。 GSE影响与认知障碍有关的蛋白质的事实表明，GSE可能通过维持神经系统的整体生存力而影响精神活性药物的作用。

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