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首页> 外文期刊>Life sciences >IDENTIFICATION OF A HUMAN HEPATOCELLULAR CARCINOMA-ASSOCIATED TUMOR SUPPRESSOR GENE BY DIFFERENTIAL DISPLAY POLYMERASE CHAIN REACTION
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IDENTIFICATION OF A HUMAN HEPATOCELLULAR CARCINOMA-ASSOCIATED TUMOR SUPPRESSOR GENE BY DIFFERENTIAL DISPLAY POLYMERASE CHAIN REACTION

机译：通过差异显示聚合酶链反应鉴定人肝细胞癌相关的肿瘤抑制基因

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Differential gene expression between the normal human liver and a cell line derived from human hepatocellular carcinoma (HCC) was studied using the differential display polymerase; chain reaction technique. One gene (mitochondria proteolipid like gene, MPL), whose expression was found to be repressed in the HCC cell line compared to normal liver, was cloned and sequenced. Amino acid sequence translated from the nucleotide sequence had a 73% homology with the carboxyl terminus of a mitochondria proteolipid (MPLP) isolated from beef heart. Northern blot analysis showed that the expression of the 3 kb MPL transcript was undetectable in 20 of 45 (44%) of human hepatocellular carcinomas, whereas only 1 of 14 (17%) of cirrhotic livers without HCC had undetectable expression when compared to normal livers. Hence MPL may be a candidate tumor suppressor gene for human HCC. This decrease in MPL expression was not due to gross alteration of its genomic DNA. [References: 49]
机译：使用差异显示聚合酶研究了正常人肝脏和人肝细胞癌（HCC）细胞系之间的差异基因表达。连锁反应技术。克隆并测序了一种基因（线粒体蛋白脂质样基因，MPL），该基因的表达在HCC细胞系中比正常肝脏受到抑制。从核苷酸序列翻译的氨基酸序列与从牛肉心分离的线粒体蛋白脂（MPLP）的羧基末端具有73％的同源性。 Northern印迹分析显示，在45个人中（20％）（44％）人类肝细胞癌中检测不到3kb MPL转录子的表达，而与正常肝脏相比，没有HCC的肝硬化肝脏中只有14个人（17％）中没有检测到表达。 。因此，MPL可能是人类HCC的候选肿瘤抑制基因。 MPL表达的下降不是由于其基因组DNA的总体改变。 [参考：49]

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