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首页> 外文期刊>Cell and Tissue Research >In situ hybridization analysis of the temporospatial expression of the midkine/pleiotrophin family in rat embryonic pituitary gland
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In situ hybridization analysis of the temporospatial expression of the midkine/pleiotrophin family in rat embryonic pituitary gland

机译：大鼠胚胎垂体中生因子/促卵磷脂家族颞pat表达的原位杂交分析

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摘要

Pituitary gland development is controlled by numerous signaling molecules, which are produced in the oral ectoderm and diencephalon. A newly described family of heparin-binding growth factors, namely midkine (MK)/pleiotrophin (PTN), is involved in regulating the growth and differentiation of many tissues and organs. Using in situ hybridization with digoxigenin-labeled cRNA probes, we detected cells expressing MK and PTN in the developing rat pituitary gland. At embryonic day 12.5 (E12.5), MK expression was localized in Rathke's pouch (derived from the oral ectoderm) and in the neurohypophyseal bud (derived from the diencephalon). From E12.5 to E19.5, MK mRNA was expressed in the developing neurohypophysis, and expression gradually decreased in the developing adenohypophysis. To characterize MK-expressing cells, we performed double-staining of MK mRNA and anterior pituitary hormones. At E19.5, no MK-expressing cells were stained with any hormone. In contrast, PTN was expressed only in the neurohypophysis primordium during all embryonic stages. In situ hybridization clearly showed that MK was expressed in primitive (immature/undifferentiated) adenohypophyseal cells and neurohypophyseal cells, whereas PTN was expressed only in neurohypophyseal cells. Thus, MK and PTN might play roles as signaling molecules during pituitary development.
机译：垂体的发育受口腔外胚层和间脑中产生的多种信号分子的控制。肝素结合生长因子家族的一个新描述，即中期因子（MK）/促卵磷脂（PTN），参与调节许多组织和器官的生长和分化。使用与洋地黄毒苷标记的cRNA探针进行原位杂交，我们在发育中的大鼠垂体腺中检测到表达MK和PTN的细胞。在胚胎第12.5天（E12.5），MK表达位于Rathke的囊袋（来自口腔外胚层）和神经下垂体芽（源自间脑）中。从E12.5到E19.5，MK mRNA在发育中的神经垂体中表达，表达在发育中的腺垂体中逐渐降低。为了表征表达MK的细胞，我们对MK mRNA和垂体前叶激素进行了双重染色。在E19.5时，没有MK表达细胞被任何激素染色。相反，在所有胚胎阶段，PTN仅在神经垂体原基中表达。原位杂交清楚地表明，MK在原始（未成熟/未分化）腺垂体细胞和神经垂体细胞中表达，而PTN仅在神经垂体细胞中表达。因此，MK和PTN可能在垂体发育过程中起信号分子的作用。

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