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Study on therapeutic action of bone marrow derived mesenchymal stem cell combined with vitamin e against acute kidney injury in rats

机译：骨髓间充质干细胞联合维生素E对大鼠急性肾损伤的治疗作用研究

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Aims: The study aims to investigate the effect to treat acute kidney injury (AKI) with bone marrow derived mesenchymal stem cells (BMSCs) combined with vitamin E and to develop a new treatment mode for AKI preclinical study. Main methods BMSCs were separated from rat bone marrow. Gentamicin was used as a damage factor in the culture of renal tubular epithelial cells (RTECs) in vitro. After co-cultured with BMSCs and vitamin E, cell proliferation of each group was detected with CCK-8. In vivo, BMSCs (3.3 × 106 cells/kg) combined with vitamin E (80 mg/kg) were administered in AKI rats induced by gentamicin intravenously. The pathological changes, biochemical parameters and apoptosis genes after treatment were investigated furthermore. Key findings In co-cultured system, proliferating ability of RTECs was improved by BMSCs or vitamin E, especially for the combined group (P 0.05). The treated rats in combined group presented the lowest serum creatinine and the highest urea nitrogen compared to non-treated rats. The improvement in renal pathological changes was followed by less necrosis, degeneration and expansion of renal tubule. Under transmission electron microscope, unclear cell structure and reduction of endoplasmic reticulum in the cytoplasm of RTECs were ameliorated with the treatment. Most apoptosis genes were up-regulated in model group while down-regulated with the therapy. Further analysis showed that the two treatments may act independently with each other. Significance Our data demonstrated that both BMSC and vitamin E hold therapeutic action to AKI induced by gentamicin. Especially, the combined treatment is better than BMSC or vitamin E alone.
机译：目的：该研究旨在探讨骨髓间充质干细胞（BMSCs）与维生素E联合治疗急性肾损伤（AKI）的效果，并为AKI临床前研究开发新的治疗模式。主要方法从大鼠骨髓中分离骨髓间充质干细胞。庆大霉素被用作体外肾小管上皮细胞（RTECs）培养的损伤因子。与BMSCs和维生素E共培养后，用CCK-8检测各组的细胞增殖。在体内，将庆大霉素静脉注射BMSCs（3.3×106细胞/ kg）与维生素E（80 mg / kg）联合给药。进一步研究治疗后的病理变化，生化指标和凋亡基因。主要发现在共培养系统中，BMSCs或维生素E改善了RTECs的增殖能力，特别是对于合并组（P <0.05）。与未治疗的大鼠相比，联合治疗组的大鼠血清肌酐最低，尿素氮最高。肾脏病理变化改善后，坏死，肾小管变性和扩张减少。在透射电镜下，通过处理可改善RTECs细胞结构不清楚和内质网减少。模型组大多数凋亡基因上调而治疗下调。进一步的分析表明，两种治疗可能彼此独立起作用。意义我们的数据表明BMSC和维生素E对庆大霉素诱导的AKI均具有治疗作用。特别是，联合治疗优于单独的BMSC或维生素E。

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