• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Peripheral type benzodiazepine receptor in T lymphocyte rich preparation.
【24h】

Peripheral type benzodiazepine receptor in T lymphocyte rich preparation.

机译：外周型苯并二氮杂receptor受体在富含T淋巴细胞的制剂中。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Some types of mood disorders and drugs are suggested to affect peripheral type benzodiazepine receptors (PBR), but their mechanisms are unclear. The isolation of pure lymphocytes is requisite for the investigation of the function of PBR on lymphocytes, since platelets and monocytes also have many PBR. The objective of this study was to establish a method of binding assay for PBR using pure T lymphocytes. Mononuclear cells and T lymphocytes were prepared by using a density gradient material and magnetic beads, respectively. The cells were analyzed using flow cytometry and a counting chamber. Binding studies were performed using T lymphocytes from 10 normal volunteers. The T lymphocytes were incubated with [3H]PK11195, harvested on glass fiber filters, and counted with a plate scintillation counter. The binding data were analyzed by the Scatchard method. With the magnetic bead technique, pure T cells were selected that contained only 1.5% monocytes and platelet/cell ratio of 1.4. The Scatchard plot of the data indicated that only one type of specific binding site was involved in the binding. The dissociation constant (Kd) was 3.8+/-1.3nM (mean+/-SD), and the Bmax was 379+/-124 fmol//10(6) cells (mean+/-SD). The density gradient- magnetic beads technique can be used as an appropriate method of preparation of T cells for PBR binding assay.
机译：建议某些类型的情绪障碍和药物会影响外周型苯并二氮杂receptor受体（PBR），但其机制尚不清楚。由于血小板和单核细胞也具有许多PBR，因此分离纯淋巴细胞是研究PBR对淋巴细胞功能的必要条件。这项研究的目的是建立使用纯T淋巴细胞的PBR结合测定方法。分别使用密度梯度材料和磁珠制备单核细胞和T淋巴细胞。使用流式细胞仪和计数室分析细胞。使用来自10名正常志愿者的T淋巴细胞进行结合研究。将T淋巴细胞与[3H] PK11195孵育，收集在玻璃纤维滤器上，并用平板闪烁计数器计数。通过Scatchard方法分析结合数据。通过磁珠技术，选择仅包含1.5％单核细胞和1.4的血小板/细胞比率的纯T细胞。数据的斯卡查德图表明结合中仅涉及一种类型的特异性结合位点。解离常数（Kd）为3.8 +/- 1.3nM（平均值+/- SD），Bmax为379 +/- 124 fmol // 10（6）细胞（平均值+/- SD）。密度梯度磁珠技术可用作制备PBR结合测定T细胞的合适方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号