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Cell adhesion molecule 1 is a new osteoblastic cell adhesion molecule and a diagnostic marker for osteosarcoma

机译：细胞粘附分子1是一种新的成骨细胞粘附分子，是骨肉瘤的诊断标记

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Aims: An immunohistochemical screen for mouse embryos showed that cell adhesion molecule 1 (CADM1), which is an immunoglobulin superfamily member, was expressed in developing bones. Here, we determined the cell types expressing CADM1 and examined its usefulness in the differential diagnosis of osteosarcoma. Main methods: Serial sections of murine developing mandibles were stained with anti-CADM1 antibody, by a coloring substrate reactive to alkaline phosphatase (ALP), a broad osteoblastic marker for preosteoblasts to osteoblasts, and by in situ hybridization for osteopontin (OPN), a marker for mature osteoblasts. CADM1 immunohistochemistry was also performed on human remodeling bones, osteosarcomas and other soft tissue tumors. Key findings: CADM1 immunohistochemistry for the mandible revealed that morphologically identifiable osteoblasts expressed CADM1 on their plasma membranes, but neither osteocytes nor bone lining cells did. At the mandibular margin, not only OPN-positive cells but also OPN-negative, ALP-positive cells were CADM1-positive, whereas inside the mandible, OPN-positive cells were often CADM1-negative. Clear membranous staining was detected in the majority of osteosarcomas (46/57), whereas only 13% (6/46) of the other soft tissue tumors were CADM1-positive (P < 0.001). Significance: These results indicated that CADM1 was a novel osteoblastic adhesion molecule that is expressed transiently during osteoblastic maturation, and a useful diagnostic marker for osteosarcoma cells.
机译：目的：对小鼠胚胎的免疫组织化学检查显示，细胞黏附分子1（CADM1）是免疫球蛋白超家族成员，在发育中的骨骼中表达。在这里，我们确定了表达CADM1的细胞类型，并检查了其在骨肉瘤鉴别诊断中的有用性。主要方法：用抗CADM1抗体，对碱性磷酸酶（ALP）有反应性的着色底物，前成骨细胞与成骨细胞的广泛成骨细胞标志物，以及原位与骨桥蛋白（OPN）杂交，对小鼠发育中的下颌骨的连续切片进行染色。成熟成骨细胞的标志物。还对人类重塑的骨骼，骨肉瘤和其他软组织肿瘤进行了CADM1免疫组织化学。关键发现：下颌骨的CADM1免疫组织化学分析表明，形态学上可识别的成骨细胞在其质膜上表达了CADM1，但骨细胞和骨衬细胞均没有。在下颌边缘，不仅OPN阳性细胞而且OPN阴性ALP阳性细胞均为CADM1阳性，而在下颌骨内部，OPN阳性细胞通常为CADM1阴性。在大多数骨肉瘤（46/57）中检测到清晰的膜状染色，而其他软组织肿瘤中只有13％（6/46）是CADM1阳性的（P <0.001）。意义：这些结果表明CADM1是在成骨细胞成熟过程中瞬时表达的新型成骨细胞粘附分子，并且是骨肉瘤细胞的有用诊断标记。

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