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首页> 外文期刊>Life sciences >In vivo amifostine (WR-2721) prevents chemotherapy-induced apoptosis of peripheral blood lymphocytes from cancer patients.
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In vivo amifostine (WR-2721) prevents chemotherapy-induced apoptosis of peripheral blood lymphocytes from cancer patients.

机译：体内氨磷汀（WR-2721）可防止化疗诱导的癌症患者外周血淋巴细胞凋亡。

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Aim of this work was to evaluate whether in vivo amifostine (WR-2721, ethanethiol, 2-[(3-aminopropyl)amino]-,dihydrogen phosphate (ester), Ethyol) pretreatment was able to prevent the apoptosis of peripheral blood lymphocytes (PBLs) induced by cytotoxic drugs. The study included 19 patients with advanced gynaecological cancers who received neoadjuvant polychemotherapy consisting of three cycles of cysplatin, adriamycin, and cyclophosphamide. Five patients received randomly amifostine pretreatment (910 mg/m2). PBLs apoptosis was measured through flow-cytometry using two different methods: a) DNA fragmentation of PBLs cultured in vitro for one hour; b) measurement of early apoptotic cells through Apostain uptake by fresh PBLs. The percentage of apoptotic PBLs was increased in all patients 24 hr after the first chemotherapy cycle (27.1 +/- 15.6 vs 6.3 +/- 6.2, p<.0001). A similar increase was observed in the following chemotherapy cycle. Amifostine pretreatment prevented the apoptosis of PBLs induced by chemotherapeutic drugs. Amifostine also prevented the reduction of lymphocyte number determined by chemotherapy. The results demonstrate that amifostine protects peripheral lymphocytes from the apoptotic damage induced by chemotherapy. This effect may explain the mechanism by which amifostine prevents the chemotherapy-associated reduction of leukocyte number.
机译：这项工作的目的是评估体内氨磷汀（WR-2721，乙硫醇，2-[（（3-氨基丙基）氨基]-，磷酸二氢酯（酯），乙醇）预处理是否能够预防外周血淋巴细胞的凋亡（ PBLs）由细胞毒性药物诱导。该研究纳入了19名患有晚期妇科癌症的患者，他们接受了新辅助化疗，包括三周期的顺铂，阿霉素和环磷酰胺治疗。五名患者接受了氨磷汀随机治疗（910 mg / m2）。通过流式细胞术使用两种不同方法测量PBL的凋亡：a）体外培养一小时的PBL的DNA片段化; b）通过新鲜PBL的Apostain摄取来测量早期凋亡细胞。所有患者在第一个化疗周期后24小时凋亡PBL的百分比均增加（27.1 +/- 15.6与6.3 +/- 6.2，p <.0001）。在随后的化疗周期中观察到类似的增加。氨磷汀预处理可防止化疗药物诱导的PBL凋亡。氨磷汀还可以防止化学疗法确定的淋巴细胞数量减少。结果表明，氨磷汀可保护外周血淋巴细胞免受化学疗法诱导的凋亡损害。这种作用可能解释了氨磷汀预防与化疗相关的白细胞数量减少的机制。

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