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首页> 外文期刊>Life sciences >Hemolysate activates P21RAS in rabbit basilar artery.
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Hemolysate activates P21RAS in rabbit basilar artery.

机译：溶血产物激活兔基底动脉中的P21RAS。

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摘要

Cerebral vasospasm is the major factor of mortality and morbidity in the patients who have an aneurysmal subarachnoid hemorrhage (SAH). Erythrocyte lysate (hemolysate), oxyhemoglobin (OxyHb), and bloody cerebrospinal fluid (CSF) are the causative agents for vasospasm. However, the signal transduction pathways for the action of these spasmogens are not clear. In this study, we examined the possible effect of these spasmogens on the p21Ras protein, an important factor in the signal cascade, in rabbit basilar artery. Hemolysate enhanced p21Ras precipitation over a 7-day period. The initial increase of p21Ras precipitation occurred after the tissues were incubated for 2 days with hemolysate. The peak effect of hemolysate, which was markedly increased compared with control (P<0.05, ANOVA), was observed on day 3. OxyHb and blood CSF, in contrast, failed to produce consistent or marked changes in p21Ras precipitation. p21Ras inhibitors FTPase inhibitor 1 and manumycin abolished hemolysate-induced enhancement of p21Ras immunoprecipitation. Genistein, a tyrosine kinase inhibitor, failed to reduce the effect of hemolysate on p21Ras. We concluded that hemolysate activates p21Ras in the rabbit basilar artery.
机译：脑血管痉挛是患有动脉瘤性蛛网膜下腔出血（SAH）的患者死亡率和发病率的主要因素。红细胞裂解物（溶血产物），氧合血红蛋白（OxyHb）和血性脑脊髓液（CSF）是引起血管痉挛的病因。但是，这些痉挛的作用的信号转导途径尚不清楚。在这项研究中，我们检查了这些痉挛源对兔基底动脉p21Ras蛋白（信号级联中的重要因素）的可能作用。溶血产物在7天的时间内增强了p21Ras的沉淀。 p21Ras沉淀的最初增加发生在组织与溶血产物温育2天后。在第3天观察到与对照相比，溶血产物的峰值作用显着增强（P <0.05，ANOVA），相反，OxyHb和血液CSF未能在p21Ras沉淀中产生一致或明显的变化。 p21Ras抑制剂FTPase抑制剂1和manumycin取消了溶血产物诱导的p21Ras免疫沉淀的增强。 Genistein，酪氨酸激酶抑制剂，未能降低溶血产物对p21Ras的作用。我们得出的结论是，溶血产物激活了兔基底动脉中的p21Ras。

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