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首页> 外文期刊>Cell cycle >Differential subcellular localizations of two human Sgo1 isoforms: implications in regulation of sister chromatid cohesion and microtubule dynamics.
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Differential subcellular localizations of two human Sgo1 isoforms: implications in regulation of sister chromatid cohesion and microtubule dynamics.

机译：两种人类Sgo1亚型的亚细胞定位差异：对姐妹染色单体内聚力和微管动力学调节的影响。

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摘要

Sgo1 is an evolutionarily conserved protein that functions as a protector of centromeric cohesin during mitosis. Recent studies show that Sgo1 is kinetochorelocalized and required for accurate segregation of mitotic chromosomes because depletion of Sgo1 in mammalian cells results in precocious initiation of anaphase and mis-segregation of chromosomes. Through analysis of GFP fusion proteins, we observe that two major isoforms of human Sgo1 exhibit entirely different subcellular localization patterns. The short isoform of Sgo1 (sSgo1) that lacks exon 6 does not localize to kinetochores during any stages of the cell cycle. Instead, it is enriched at mitotic spindles. On the other hand, the longer isoform of Sgo1 primarily localizes to kinetochores during G(2) phase and mitotic prophase, metaphase, and anaphase. During late mitosis, Sgo1 does not appear to be associated with kinetochores. Intriguingly, the longer isoform of Sgo1 forms discrete foci during S phase, some of which are apparently in the nucleoli. However, a majority of these foci colocalize with CREST, a kinetochore antigen, indicating that Sgo1 is loaded onto kinetochores during or immediately after DNA replication. Together, our studies suggest that different isoforms of Sgo1 may play distinct roles during the cell cycle and that Sgo1 may have an interphase function as well.
机译：Sgo1是一种进化保守的蛋白质，在有丝分裂期间充当着丝粒粘着蛋白的保护剂。最近的研究表明，Sgo1是动线粒体定位的，并且是有丝分裂染色体精确分离所必需的，因为哺乳动物细胞中Sgo1的耗尽会导致后期成熟和染色体的错误分离。通过分析GFP融合蛋白，我们观察到人类Sgo1的两个主要亚型表现出完全不同的亚细胞定位模式。缺少外显子6的Sgo1（sSgo1）的短同种型在细胞周期的任何阶段都不会定位到动植物。相反，它在有丝分裂纺锤体上富集。另一方面，Sgo1的较长同工型主要定位在G（2）阶段和有丝分裂前期，中期和后期的动植物。在有丝分裂晚期，Sgo1似乎与动植物无关。有趣的是，Sgo1的较长同工型在S期形成离散的病灶，其中一些显然在核仁​​中。但是，这些病灶中的大多数与CREST（一种线粒体抗原）共定位，表明Sgo1在DNA复制过程中或复制后立即被加载到动植物上。在一起，我们的研究表明Sgo1的不同同工型可能在细胞周期中发挥不同的作用，并且Sgo1也可能具有相间功能。

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