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首页> 外文期刊>Cellular immunology >Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues.
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Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues.

机译：肝表达的抗菌肽2（LEAP-2）变体形式在人体组织中的表达和功能分析。

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The antimicrobial peptide Liver Expressed Antimicrobial Peptide-2 (LEAP-2) is proposed to function as part of the vertebrate innate immune system. However, the highly conserved nature of the LEAP-2 peptide primary structure among vertebrates suggests more fundamental physiological roles. RT-PCR analyses confirmed expression of LEAP-2 mRNA variants in human gastro-intestinal (GI) epithelial tissues and THP-1 monocytes. Three cDNA products indicative of at least three different spliced transcripts were observed. Translation of the cDNA sequences supported synthesis of transcripts encoding the secreted LEAP-2 peptide and two variants lacking signal sequences suggesting intracellular localisation. The synthesis and cytoplasmic localisation of LEAP-2 peptides in epithelia was supported by immunohistochemical analyses. Functional data suggested that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. However, changes in the LEAP-2 transcript patterns associated with the challenge of THP-1 monocytes with lipopolysaccharide (100ng/ml) were supportive of the peptides having multiple roles in the innate immune response.
机译：有人建议将抗菌肽肝表达抗菌肽2（LEAP-2）用作脊椎动物先天免疫系统的一部分。但是，脊椎动物中LEAP-2肽一级结构的高度保守性质提示其更基本的生理作用。 RT-PCR分析证实了LEAP-2 mRNA变体在人胃肠道（GI）上皮组织和THP-1单核细胞中的表达。观察到指示至少三个不同剪接转录物的三个cDNA产物。 cDNA序列的翻译支持编码分泌的LEAP-2肽的转录物的合成以及缺少暗示细胞内定位的信号序列的两个变体。免疫组织化学分析支持LEAP-2肽在上皮细胞中的合成和细胞质定位。功能数据表明，LEAP-2不参与GI上皮对铁的生理反应，对上皮细胞也没有促有丝分裂作用，对THP-1单核细胞也没有趋化作用。但是，与THP-1单核细胞受到脂多糖（100ng / ml）攻击有关的LEAP-2转录模式变化支持了在先天免疫应答中具有多种作用的肽。

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