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首页> 外文期刊>Radiation Research: Official Organ of the Radiation Research Society >Biophysical modeling of fragment length distributions of DNA plasmids after X and heavy-ion irradiation analyzed by atomic force microscopy
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Biophysical modeling of fragment length distributions of DNA plasmids after X and heavy-ion irradiation analyzed by atomic force microscopy

机译：X射线和重离子辐照后通过原子力显微镜分析DNA质粒片段长度分布的生物物理模型

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The investigation of fragment length distributions of plasmid DNA gives insight into the influence of localized energy distribution on the induction of DNA damage, particularly the clustering of double-strand breaks. We present an approach that determines the fragment length distributions of plasmid DNA after heavy-ion irradiation by using the Local Effect Model. We find a good agreement of our simulations with experimental fragment distributions derived from atomic force microscopy (AFM) studies by including experimental constraints typical for AFM. Our calculations reveal that by comparing the fragmentation in terms of fluence, we can uniquely distinguish the effect of different radiation qualities. For very high-LET irradiation using nickel or uranium ions, no difference between their fragment distributions can be expected for the same dose level. However, for carbon ions with an intermediate LET, the fragmentation pattern differs from the distribution for very high-LET particles. The results of the model calculations can be used to determine the optimal experimental parameters for a demonstration of the influence of track structure on primary radiation damage. Additionally, we compare the results of our model for two different plasmid geometries. (C) 2008 Radiation Research Society.
机译：质粒DNA片段长度分布的研究可以深入了解局部能量分布对DNA损伤诱导的影响，特别是双链断裂的聚集。我们提出一种使用局部效应模型确定重离子辐照后质粒DNA片段长度分布的方法。通过包含原子力显微镜的典型实验约束条件，我们发现我们的模拟与原子力显微镜（AFM）研究得出的实验碎片分布非常吻合。我们的计算表明，通过比较通量方面的碎片，我们可以唯一地区分不同辐射质量的影响。对于使用镍或铀离子进行的非常高的LET辐射，在相同剂量水平下，其碎片分布之间不会有差异。但是，对于具有中等LET的碳离子，其碎裂模式与非常高LET的颗粒的分布不同。模型计算的结果可用于确定最佳实验参数，以证明轨道结构对主要辐射损伤的影响。此外，我们比较了两种不同质粒几何形状的模型结果。 （C）2008辐射研究学会。

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