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首页> 外文期刊>Radiation Research: Official Organ of the Radiation Research Society >PRODUCTION OF DNA DOUBLE-STRAND BREAKS BY INTERACTIONS BETWEEN CARBOPLATIN AND RADIATION - A POTENTIAL MECHANISM FOR RADIOPOTENTIATION
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PRODUCTION OF DNA DOUBLE-STRAND BREAKS BY INTERACTIONS BETWEEN CARBOPLATIN AND RADIATION - A POTENTIAL MECHANISM FOR RADIOPOTENTIATION

机译：碳白蛋白与辐射相互作用产生DNA双链断裂-潜在的放射增敏机制

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The production of DNA double-strand breaks (DSBs) was studied in cells of four CHO cell lines under conditions where combining radiation with carboplatin enhanced cell killing (radiosensitization and radiopotentiation). The cell lines included repair-proficient (AA8 and K1), excision repair-deficient (UV41) and DSB repair-deficient (xrs-5) cells. Double-strand breaks were analyzed by neutral elution either immediately after or 4 h after a single 55-Gy radiation dose delivered under hypoxic conditions. Carboplatin (1 mM) combined with radiation produced a small increase in DSBs compared to radiation alone immediately after irradiation in AA8, UV41 and xrs-5 cells. However, the yield of DSBs in AA8, K1 and xrs-5 cells was significantly higher 4 h after carboplatin-radiation treatment; no such increase was found at 4 h in UV41 cells. Strand scission factors (SSFs) were calculated as SSF = -log [percentage DNA remaining on filter at 8 h elution (treated cells)/percentage DNA remaining at 8 h elution (untreated cells)]. The ratios of the SSF at 4 h to 0 h postirradiation for carboplatin-treated cells were 13.7 for K1, 4.9 for xrs-5, 2.5 for AA8 and 1.2 for UV41 cells. These results support a possible explanation for the enhanced killing of irradiated cells by platinum chemotherapeutic agents, namely enhanced production of DSBs. (C) 1995 by Radiation Research Society [References: 27]
机译：在结合放射线和卡铂增强细胞杀伤（放射增敏和放射增效）的条件下，研究了四个CHO细胞系细胞中DNA双链断裂（DSB）的产生。细胞系包括修复能力强的细胞（AA8和K1），切除修复缺陷的细胞（UV41）和DSB修复缺陷的细胞（xrs-5）。在缺氧条件下递送单次55 Gy辐射剂量后或之后4小时，通过中性洗脱分析双链断裂。与在AA8，UV41和xrs-5细胞中照射后立即单独照射相比，卡铂（1 mM）与照射相结合产生的DSB略有增加。然而，卡铂辐射处理后4 h，AA8，K1和xrs-5细胞中DSB的产量显着提高。在UV41细胞中在4小时没有发现这种增加。链断裂因子（SSF）计算为SSF ＝ -log [在8小时洗脱时保留在过滤器上的DNA百分比（处理过的细胞）/在8小时洗脱时保留在过滤器上的百分比DNA（未处理的细胞）]。接受卡铂处理的细胞在照射后4 h至0 h的SSF比率对于K1为13.7，对于xrs-5为4.9，对于AA8为2.5，对于UV41细胞为1.2。这些结果支持了铂化学治疗剂对辐射细胞杀伤力增强的可能解释，即DSBs产量提高。 （C）1995年，辐射研究学会[参考文献：27]

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