CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: role of beta(1) (CD29)-, beta(2) (CD18)-related integrins and non-integrin receptors.

Shenkman B; Brill G; Solpov A; Vitkovsky Y; Kuznik B; Koltakov A; Kotev-Emeth S; Savion N; Bank I

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CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: role of beta(1) (CD29)-, beta(2) (CD18)-related integrins and non-integrin receptors.

机译：CD4 +淋巴细胞需要血小板与流动中的固定纤连蛋白粘附：β（1）（CD29）-，β（2）（CD18）相关整联蛋白和非整联蛋白受体的作用。

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The role of platelets in T-lymphocytes adhesion is not clear yet. Herpesvirus saimiri (HVS)-infected CD4(+) T-lymphocytes were placed into polystyrene plates pre-coated with fibronectin. The adherent T-cells were enumerated by image analysis. Under static condition, 38+/-10cells/mm(2) adhered and addition of gel-filtered platelets (GFP) and PMA enhanced cell adhesion 4.3- and 4.1-fold. Using PMA plus GFP 11.9-fold enhancement in cell adhesion was achieved. In contrast, under flow (200s(-1)), neither basal adhesion nor following separate addition of PMA or GFP was observed, whereas combined addition of PMA and GFP induced noticeable adhesion (34cells/mm(2)). The adhesion was inhibited by blockade of alpha(5)-integrin (CD49e, 87%), beta(2)-integrin (CD18, 78%), CD40L (60%), PSGL-1 (CD162, 60%), and CD40L plus PSGL-1 (83%). Thus, activated platelets promote activated T-cell adhesion to fibronectin under flow via integrins (alpha(5)beta(1), and alpha(L)beta(2)), CD40-CD40L and P-selectin-PSGL-1 mediated interactions.

机译：血小板在T淋巴细胞粘附中的作用尚不清楚。将感染疱疹病毒（HVS）的CD4（+）T淋巴细胞放入预先涂有纤连蛋白的聚苯乙烯平板中。通过图像分析枚举粘附的T细胞。在静态条件下，粘附38 +/- 10cells / mm（2），并且添加凝胶过滤的血小板（GFP）和PMA可以增强细胞粘附力4.3倍和4.1倍。使用PMA和GFP，细胞粘附力提高了11.9倍。相比之下，在流动（200s（-1））下，既没有观察到基底粘附，也没有观察到分别添加PMA或GFP，而同时加入PMA和GFP则引起明显的粘附（34cells / mm（2））。粘附被α（5）-整合素（CD49e，87％），β（2）-整合素（CD18，78％），CD40L（60％），PSGL-1（CD162，60％）和CD40L加PSGL-1（83％）。因此，活化的血小板通过整合素（alpha（5）beta（1）和alpha（L）beta（2）），CD40-CD40L和P-selectin-PSGL-1介导的相互作用促进活化的T细胞粘附于纤连蛋白。

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来源
《Cellular immunology》 |2006年第1期|共8页
作者
Shenkman B; Brill G; Solpov A; Vitkovsky Y; Kuznik B; Koltakov A; Kotev-Emeth S; Savion N; Bank I;
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正文语种 eng
中图分类细胞生物学;医学免疫学;
关键词
Antigens; CD18/me Metabolism; Antigens; CD29/me Metabolism; *Blood Platelets/me Metabolism; *CD4-Positive T-Lymphocytes/me Metabolism; CD4-Positive T-Lymphocytes/vi Virology; *Cell Adhesion/ph Physiology; *Cell Communication/ph Physiology; Extracellular Matrix/me Metabolism; *Fibronectins/me Metabolism; Herpesvirus 2; Saimiriine; Humans; Immunomagnetic Separation; Lymphocyte Activation/ph Physiology;

机译：抗原;CD18 / me [代谢];抗原;CD29 / me [代谢];*血小板/ me [代谢];* CD4阳性T淋巴细胞/ me [代谢];CD4阳性T淋巴细胞/ vi [病毒学] ];*细胞粘附力/ ph [生理学];*细胞通讯/ ph [生理学];细胞外基质/ me [代谢];*纤连蛋白/ me [代谢];疱疹病毒2;Saimiriine;人类;免疫磁分离;淋巴细胞活化/ ph [生理];

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1. CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: role of beta(1) (CD29)-, beta(2) (CD18)-related integrins and non-integrin receptors. [J] . Shenkman B, Brill G, Solpov A, Cellular immunology . 2006,第1期

机译：CD4 +淋巴细胞需要血小板与流动中的固定纤连蛋白粘附：β（1）（CD29）-，β（2）（CD18）相关整联蛋白和非整联蛋白受体的作用。
2. CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: role of beta(1) (CD29)-, beta(2) (CD18)-related integrins and non-integrin receptors. [J] . Shenkman B, Brill G, Solpov A, Cellular immunology . 2006,第1期

机译：CD4 +淋巴细胞需要血小板与流动中的固定纤连蛋白粘附：β（1）（CD29）-，β（2）（CD18）相关整联蛋白和非整联蛋白受体的作用。
3. Platelets enhance CD4+ lymphocyte adhesion to extracellular matrix under flow conditions: role of platelet aggregation, integrins, and non-integrin receptors. [J] . Solpov A, Shenkman B, Vitkovsky Y, Thrombosis and Haemostasis: Journal of the International Society on Thrombosis and Haemostasis . 2006,第5期

机译：血小板在流动条件下增强CD4 +淋巴细胞对细胞外基质的粘附：血小板聚集，整联蛋白和非整联蛋白受体的作用。
4. Characterization of Fibronectin Assembly by Adherent Platelets Under Flow Conditions: Effect of Shear Stress and Role of B3 Integrins [C] . Khon Huynh, Phong Le, Thao Nguyen International Conference on the Development of Biomedical Engineering in Vietnam . 2018

机译：流动条件下粘附血小板纤连蛋白组装的表征：剪切应力的影响和B3整合蛋白的作用
5. Monocyte priming during dyslipidemia: Elevated beta2-integrin CD11c/CD18 orchestrates enhanced adhesion to VCAM-1. [D] . Gower, Robert Michael. 2010

机译：血脂异常时单核细胞启动：升高的β2-整合素CD11c / CD18可以增强与VCAM-1的粘附力。
6. Polymorphonuclear leucocyte migration through human dermal fibroblast monolayers is dependent on both beta 2-integrin (CD11/CD18) and beta 1-integrin (CD29) mechanisms. [O] . J X Gao, A C Issekutz 1995

机译：通过人皮肤成纤维细胞单层的多形核白细胞迁移既依赖于β2整合素（CD11 / CD18）也依赖于β1整合素（CD29）机制。
7. Beta 2 (CD18) and beta 1 (CD29) integrin mechanisms in migration of human polymorphonuclear leucocytes and monocytes through lung fibroblast barriers: shared and distinct mechanisms. [O] . Shang, X Z, Issekutz, A C 1997

机译：Beta 2（CD18）和beta 1（CD29）整合素在人类多形核白细胞和单核细胞通过肺成纤维细胞屏障迁移中的机制：共享和独特的机制。

CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: role of beta(1) (CD29)-, beta(2) (CD18)-related integrins and non-integrin receptors.

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