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首页> 外文期刊>Cellular immunology >Comparative proteome analysis of splenic lymphocytes in long-term high-fat diet and dietary supplement with lipoic acid mice.
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Comparative proteome analysis of splenic lymphocytes in long-term high-fat diet and dietary supplement with lipoic acid mice.

机译：长期高脂饮食和硫辛酸小鼠饮食补充物中脾淋巴细胞的比较蛋白质组分析。

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摘要

The objective of this investigation was to explore possible molecular changes for role of a high-fat diet (HFD)-induced oxidative stress in splenic lymphocytes, and whether a dietary lipoic acid (LA) supplement could attenuate these changes. Male C57BL/6 mice were fed one of three diets 10 weeks and outcome measures centered on parameters of oxidative stress and lymphocytes apoptosis in spleen. Two-dimensional gel electrophoresis was used to compare the proteomes of splenic lymphocytes with three dietary groups. Differentially expressed spots whose expression altered over three fold were identified by MALDI-TOF MS. In this study, HFD resulted in oxidative stress in mice spleen, and significantly increased apoptotic percentage of splenic lymphocytes. Bioinformatic evaluation results of MALDI-TOF MS showed that 20 differentially expressed protein spots were known to be involved in many processes associated with cell function, such as cytoskeleton, energy metabolism and oxidative stress, signal transduction and cell defense. In conclusion, these results indicate that HFD-induced oxidative stress could lead to the functional decline of splenic lymphocytes, and LA supplement attenuates the alterations of protein expression to maintain the basic biological processes.
机译：这项研究的目的是探讨可能的分子变化对脾脏淋巴细胞中高脂饮食（HFD）诱导的氧化应激的作用，以及膳食硫辛酸（LA）补充剂是否可以减轻这些变化。雄性C57BL / 6小鼠在10周内接受了三种饮食之一的喂养，其结局指标集中在氧化应激和脾脏淋巴细胞凋亡的参数上。二维凝胶电泳用于比较三个饮食组的脾淋巴细胞的蛋白质组。通过MALDI-TOF MS鉴定了表达变化超过三倍的差异表达斑点。在这项研究中，HFD导致小鼠脾脏氧化应激，并显着增加脾淋巴细胞的凋亡百分比。 MALDI-TOF MS的生物信息学评估结果表明，已知有20个差异表达的蛋白斑点参与了许多与细胞功能相关的过程，例如细胞骨架，能量代谢和氧化应激，信号转导和细胞防御。总之，这些结果表明，HFD诱导的氧化应激可能导致脾淋巴细胞的功能下降，而LA补充剂可减弱蛋白质表达的变化，从而维持基本的生物学过程。

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