Autocrine Production of Insulin-like Growth Factor-I (IGF-I) Affects Paracellular Transport Across Epithelial Cells In Vitro

Julie M. D. Paye; R. Michael Akers; William R. Huckle; Kimberly Forsten-Williams

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Autocrine Production of Insulin-like Growth Factor-I (IGF-I) Affects Paracellular Transport Across Epithelial Cells In Vitro

机译：胰岛素样生长因子-I（IGF-I）的自分泌产生影响跨上皮细胞的细胞旁运输。

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Autocrine production of growth factors can have significant effects on cell activity. We report for the first time that autocrine production of insulin-like growth factor-I (IGF-I) alters paracellular transport across bovine mammary epithelial cells in vitro. Paracellular transport was assessed by measuring phenol red transport across mammary alveolar cells-large T antigen (MAC-T cells) derived from parental mammary epithelial cells, cultured on porous membranes and compared with two different transfected MAC-T cell lines that constitutively secrete IGF-I. Phenol red transport was essentially blocked in parental cell culture after six days, while IGF-I secreting cells provided essentially no barrier. Surprisingly, neither co-culture studies between parental and IGF-I-secreting cells nor addition of exogenous IGF-I or IGF-binding protein-3 reversed the phenol red transport properties. IGF-I-secreting cells did however express lower levels of the junction components occludin and E-cadherin than parental cells, suggesting that localized autocrine IGF-I activity might lead to increased permeability via changes in both the tight and adherens junction protein levels.

机译：自分泌分泌生长因子可对细胞活性产生重大影响。我们首次报道胰岛素样生长因子-I（IGF-I）的自分泌生产改变了体外牛乳腺上皮细胞的旁细胞转运。通过测量跨亲代乳腺上皮细胞的乳腺肺泡细胞-大T抗原（MAC-T细胞）中酚红的转运来评估细胞旁转运，将其培养在多孔膜上，并与组成性分泌IGF-的两种不同的转染MAC-T细胞系进行比较一世。六天后，亲代细胞培养中酚红的转运基本上被阻断，而分泌IGF-1的细胞基本上没有提供屏障。令人惊讶地，亲本和分泌IGF-I的细胞之间的共培养研究或添加外源性IGF-I或IGF-结合蛋白-3均未改变酚红转运性质。然而，分泌IGF-I的细胞确实比亲代细胞表达更低水平的连接成分occludin和E-cadherin，这表明局部自分泌IGF-I活性可能通过紧密连接蛋白和粘附连接蛋白水平的变化导致通透性增加。

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《Cell communication & adhesion》 |2007年第3期|共14页
作者
Julie M. D. Paye; R. Michael Akers; William R. Huckle; Kimberly Forsten-Williams;
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正文语种 eng
中图分类细胞生物学;
关键词
autocrine; E-cadherin; epithelial; insulin-like growth factor-I (IGF-I); IGF-I receptor (IGF-IR); IGFBP-2; IGFBP-3; MAC-T; mammary alveolar cells-large T antigen; mammary epithelial cells;

机译：自分泌;E-钙黏着蛋白;上皮;胰岛素样生长因子-I（IGF-1）;IGF-I受体（IGF-IR）;IGFBP-2;IGFBP-3;MAC-T;乳腺肺泡细胞大T抗原乳腺上皮细胞;

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专利

1. Autocrine Production of Insulin-like Growth Factor-I (IGF-I) Affects Paracellular Transport Across Epithelial Cells In Vitro [J] . Julie M. D. Paye, R. Michael Akers, William R. Huckle, Cell communication & adhesion . 2007,第2a3期

机译：胰岛素样生长因子-I（IGF-I）的自分泌产生影响跨上皮细胞的细胞旁运输。
2. Estradiol and triiodothyronine increase production of insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) by GH4C1 rat pituitary tumor cells. [J] . Gilchrist CA, Park JH, MacDonald RG, Molecular and Cellular Endocrinology . 1995,第1a2期

机译：雌二醇和三碘甲腺氨酸增加了GH4C1大鼠垂体肿瘤细胞对胰岛素样生长因子I（IGF-1）和胰岛素样生长因子结合蛋白3（IGFBP-3）的产生。
3. The insulin-like growth factor-I receptor kinase inhibitor NVP-AEW541 induces apoptosis in acute myeloid leukemia cells exhibiting autocrine insulin-like growth factor-I secretion [J] . P L Tazzari, G Tabellini, R Bortul, Leukemia . 2007,第5期

机译：胰岛素样生长因子-I受体激酶抑制剂NVP-AEW541诱导显示自分泌胰岛素样生长因子-I分泌的急性髓样白血病细胞凋亡
4. Genetic Association Study of Insulin-like Growth Factor-I (IGF-I) Gene with Curve Severity and Osteopenia in Adolescent Idiopathic Scoliosis [C] . YEUNG HY, TANG NL, LEE KM, International Research Society of Spinal Deformities . 2008

机译：胰岛素样生长因子-1（IGF-I）基因曲线严重程度和骨抑制性骨抑制症的遗传结合研究
5. Autocrine mechanisms of action of insulin-like growth factor I (IGF-I) and hormonal regulation of expression of IGF-binding proteins in mammary epithelial cells. [D] . Romagnolo, Donato. 1993

机译：胰岛素样生长因子I（IGF-I）的自分泌作用机制和乳腺上皮细胞中IGF结合蛋白表达的激素调节。
6. Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3 [O] . Noelle-Anne Sunstrom, Masood Baig, Louise Cheng, 1998

机译：Super-CHO中重组胰岛素样生长因子-I（IGF-I）的产生导致IGF-I受体和IGF结合蛋白3的表达
7. Insulin-like growth factor-I (IGF-I) antisense oligodeoxynucleotide mediated inhibition of DNA synthesis by WI-38 cells: evidence for autocrine actions of IGF-I. [O] . B M Moats-Staats, G Z Retsch-Bogart, W A Price, 1993

机译：胰岛素样生长因子-1（IGF-1）反义寡脱氧核苷酸介导的WI-38细胞DNA合成的抑制：IGF-1的自分泌作用的证据。
8. Collagen and Stretch Modulate Autocrine Secretion of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Proteins from Differentiated Skeletal Muscle Cells [R] . Perrone, C. E. , Fenwick-Smith, D. , Vandenburgh, H. H. 1995

机译：胶原蛋白和拉伸调节胰岛素样生长因子-1和胰岛素样生长因子结合蛋白从分化的骨骼肌细胞分泌自分泌

Autocrine Production of Insulin-like Growth Factor-I (IGF-I) Affects Paracellular Transport Across Epithelial Cells In Vitro

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