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首页> 外文期刊>Reproduction, fertility, and development >Incidence of chromosomal abnormalities in bovine blastocysts derived from unsorted and sex-sorted spermatozoa.
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Incidence of chromosomal abnormalities in bovine blastocysts derived from unsorted and sex-sorted spermatozoa.

机译：来自未分选和按性别分选的精子的牛胚泡中染色体异常的发生率。

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摘要

The aim of the present study was to examine the incidence of chromosomal abnormalities in bovine blastocysts produced by IVF with unsorted, X-sorted or Y-sorted spermatozoa. In Experiment 1, individual blastocysts were processed to examine the incidence of mixoploidy using fluorescent in situ hybridisation. Overall, 80% (44/55) of blastocysts were mixoploid (10/15, 14/15 and 20/25 for X-sorted, Y-sorted and unsorted spermatozoa, respectively; P > 0.05). However, the prevalence of abnormal XY chromosome complements was relatively low in all groups; on average, only a small fraction of the total nuclei per embryo appeared polyploid (1.64%, 5.62% and 6.0% for X-sorted, Y-sorted and unsorted spermatozoa, respectively). Interestingly, 20% (5/25) of blastocysts derived from unsorted spermatozoa were found to be chimeric (XX/XY). In Experiment 2, chimeric embryos were detected among the blastocysts derived from two of five sires tested. In addition, one chimeric blastocyst was detected among nine in vivo-derived blastocysts obtained following AI. In conclusion, based on the results of the present study, the incidence of chromosomal abnormalities did not different between blastocysts derived from sex-sorted or unsorted spermatozoa. In addition, the occurrence of mixed sex chimeras was not limited to a single sire and was not unique to blastocysts derived from IVF.
机译：本研究的目的是检查由未分选，X分选或Y分选的精子通过IVF产生的牛胚泡中染色体异常的发生率。在实验1中，使用荧光原位杂交对单个胚泡进行处理，以检查混合倍性的发生率。总体而言，80％（44/55）的胚泡是混合倍体的（对于X分选，Y分选和未分选的精子分别为10 / 15、14 / 15和20/25; P> 0.05）。然而，在所有组中，异常XY染色体补体的患病率都相对较低。平均而言，每个胚胎的总核中只有一小部分出现多倍体（X分选，Y分选和未分选的精子分别为1.64％，5.62％和6.0％）。有趣的是，发现20％（5/25）的未分类精子囊胚是嵌合的（XX / XY）。在实验2中，在源自五个受测父亲中的两个的囊胚中检测到嵌合胚。另外，在AI后获得的9个体内来源的胚泡中检测到1个嵌合胚泡。总之，基于本研究的结果，染色体异常的发生率在源自性别分类或未分类的精子的囊胚之间没有差异。此外，混合性嵌合体的发生不仅限于单只，也不是源自IVF的胚泡所独有。

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