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首页> 外文期刊>Cell cycle >Localization and function of mSpindly during mouse oocyte meiotic maturation.
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Localization and function of mSpindly during mouse oocyte meiotic maturation.

机译：小鼠卵母细胞减数分裂成熟过程中mSpindly的定位和功能。

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Spindly was first identified in Drosophila; its homologues are termed SPDL-1 in Caenorhabditis elegans and Hs Spindly/hSpindly in humans. In all species, Spindly and its homologues function by recruiting dynein to kinetochores and silencing SAC in mitosis of somatic cells. Depletion of Spindly causes an extensive metaphase arrest during somatic mitoses in Drosophila, C. elegans and humans. In Drosophila, Spindly is required for shedding of Rod and Mad2 from the kinetochores in metaphase; in C. elegans, SPDL-1 presides over the recruitment of dynein and MDF-1 to the kinetochores; in humans, Hs Spindly is required for recruiting both dynein and dynactin to kinetochores but it is dispensable for removal of checkpoint proteins from kinetochores. The present study was designed to investigate the localization and function of the Spindly homologue (mSpindly) during mouse oocyte meiotic maturation by immunofluorescent analysis, and by overexpression and knockdown of mSpindly. We found that mSpindly was typically localized to kinetochores when chromatin condensed into chromosomes after GVBD. In metaphase of both first meiosis and second meiosis, mSpindly was localized not only to kinetochores but also to the spindle poles. Overexpression of mSpindly did not affect meiotic progression, but its depletion resulted in an arrest of the pro-MI/MI stage, failure of anaphase entry and subsequent polar body emission, and in abnormal spindle morphology and misaligned chromosomes. Our data suggest that mSpindly participates in SAC silencing and in spindle formation as a recruiter and/or a transporter of kinetochore proteins in mouse oocytes, but that it needs to cooperate with other factors to fulfill its function.
机译：Spindly最先在果蝇中发现。其同源物在秀丽隐杆线虫中被称为SPDL-1，在人类中被称为Hs Spindly / hSpindly。在所有物种中，Spindly及其同系物通过将动力蛋白募集到动植物并使体细胞的有丝分裂中的SAC沉默而起作用。 Spindly的耗竭在果蝇，秀丽隐杆线虫和人类的体细胞有丝分裂期间引起广泛的中期停滞。在果蝇中，需要使用Spindly才能从中期的动植物中脱落杆和Mad2。在秀丽隐杆线虫中，SPDL-1负责将动力蛋白和MDF-1募集到动植物。在人类中，需要Hs Spindly才能将动力蛋白和动力蛋白募集到动植物中，但对于从动植物中去除检查点蛋白是必不可少的。本研究旨在通过免疫荧光分析以及mSpindly的过表达和敲低研究Spindly同源物（mSpindly）在小鼠卵母细胞减数分裂成熟过程中的定位和功能。我们发现，当染色质浓缩到GVBD后的染色体中时，mSpindly通常位于动粒体中。在第一次减数分裂和第二次减数分裂的中期，mSpindly不仅定位于动植物，而且定位于纺锤体极。 mSpindly的过表达并不会影响减数分裂的进程，但其耗竭会导致MI / MI前期的停止，后期进入的失败以及随后的极体发射，以及异常的纺锤体形态和染色体错位。我们的数据表明，mSpindly作为小鼠卵母细胞中动线粒蛋白的募集者和/或转运蛋白，参与了SAC沉默和纺锤体形成，但是它需要与其他因素配合才能发挥其功能。

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