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首页> 外文期刊>Radiotherapy and oncology: Journal of the European Society for Therapeutic Radiology and Oncology >Regeneration of irradiated salivary glands with stem cell marker expressing cells.
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Regeneration of irradiated salivary glands with stem cell marker expressing cells.

机译：用表达干细胞标记的细胞再生唾液腺。

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BACKGROUND: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland. METHODS: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers. RESULTS: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function. CONCLUSIONS: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland.
机译：背景：干细胞疗法可能是减少辐射引起的唾液流涎和改善患者生活质量的潜在途径。但是，唾液腺干细胞的鉴定和纯化尚未完成。这项研究旨在更好地表征小鼠唾液腺中具有再生潜能的干/祖细胞群。方法：采用免疫组织化学检测组织和流式细胞仪检测小鼠下颌下腺组织，分离的细胞和培养3天的唾液中干细胞标记c-Kit，CD133，CD49f和CD24的表达。用15 Gy的单剂量局部照射小鼠，并移植表达明确标志物的细胞。结果：表达已知干细胞标记的细胞位于小鼠唾液腺的较大导管中。分离的细胞和第3天唾液中的细胞也表达以下标记：c-Kit（0.058％vs. 0.65％），CD133（6％vs. 5％），CD49f（78％vs.51％）和CD24（60％分别为60％）。将这些细胞进行腺内移植到受辐照的小鼠唾液腺中可导致唾液腺功能恢复干细胞标记特异性。结论：小鼠唾液腺细胞表达了不同的干细胞相关标志物，这些标志物在移植后能够再生受辐射损伤的唾液腺。

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