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首页> 外文期刊>Cell cycle >Regulation of Ace2-dependent genes requires components of the PBF complex in Schizosaccharomyces pombe
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Regulation of Ace2-dependent genes requires components of the PBF complex in Schizosaccharomyces pombe

机译：Ace2依赖基因的调节需要粟酒裂殖酵母中PBF复合物的成分

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The division cycle of unicellular yeasts is completed with the activation of a cell separation program that results in the dissolution of the septum assembled during cytokinesis between the 2 daughter cells, allowing them to become independent entities. Expression of the eng1(+) and agn1(+) genes, encoding the hydrolytic enzymes responsible for septum degradation, is activated at the end of each cell cycle by the transcription factor Ace2. Periodic ace2(+) expression is regulated by the transcriptional complex PBF (PCB Binding Factor), composed of the forkhead-like proteins Sep1 and Fkh2 and the MADS box-like protein Mbx1. In this report, we show that Ace2-dependent genes contain several combinations of motifs for Ace2 and PBF binding in their promoters. Thus, Ace2, Fkh2 and Sep1 were found to bind in vivo to the eng1(+) promoter. Ace2 binding was coincident with maximum level of eng1(+) expression, whereas Fkh2 binding was maximal when mRNA levels were low, supporting the notion that they play opposing roles. In addition, we found that the expression of eng1(+) and agn1(+) was differentially affected by mutations in PBF components. Interestingly, agn1(+) was a major target of Mbx1, since its ectopic expression resulted in the suppression of Mbx1 deletion phenotypes. Our results reveal a complex regulation system through which the transcription factors Ace2, Fkh2, Sep1 and Mbx1 in combination control the expression of the genes involved in separation at the end of the cell division cycle.
机译：单细胞酵母的分裂周期通过激活细胞分离程序完成，该程序导致2个子细胞之间在胞质分裂过程中组装的隔膜溶解，从而使它们成为独立的实体。 eng1（+）和agn1（+）基因的表达（编码负责隔膜降解的水解酶）在每个细胞周期结束时被转录因子Ace2激活。 ace2（+）的周期性表达受转录复合物PBF（PCB结合因子）调控，该复合物由叉头状蛋白Sep1和Fkh2和MADS盒状蛋白Mbx1组成。在此报告中，我们表明Ace2依赖性基因在其启动子中包含Ace2和PBF结合的基序的几种组合。因此，发现Ace2，Fkh2和Sep1在体内与eng1（+）启动子结合。 Ace2绑定与eng1（+）表达的最大水平相吻合，而当mRNA水平较低时，Fkh2绑定最大，支持它们起相反作用的观点。此外，我们发现eng1（+）和agn1（+）的表达受到PBF组件突变的影响。有趣的是，agn1（+）是Mbx1的主要靶标，因为其异位表达导致Mbx1缺失表型的抑制。我们的结果揭示了一个复杂的调控系统，通过该调控系统，转录因子Ace2，Fkh2，Sep1和Mbx1共同控制细胞分裂周期结束时参与分离的基因的表达。

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