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首页> 外文期刊>Cell cycle >Stochastic exit from mitosis in budding yeast: model predictions and experimental observations.
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Stochastic exit from mitosis in budding yeast: model predictions and experimental observations.

机译：萌芽酵母中有丝分裂的随机出口：模型预测和实验观察。

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摘要

Unlike many mutants that are completely viable or inviable, the CLB2-dbDelta clb5Delta mutant of Saccharomyces cerevisiae is inviable in glucose but partially viable on slower growth media such as raffinose. On raffinose, the mutant cells can bud and divide but in each cycle there is a chance that a cell will fail to divide (telophase arrest), causing it to exit the cell cycle. This effect gives rise to a stochastic phenotype that cannot be explained by a deterministic model. We measure the inter-bud times of wild type and mutant cells growing on raffinose and compute statistics and distributions to characterize the mutant's behavior. We convert a detailed deterministic model of the budding yeast cell cycle to a stochastic model and determine the extent to which it captures the stochastic phenotype of the mutant strain. Predictions of the mathematical model are in reasonable agreement with our experimental data and suggest directions for improving the model. Ultimately, the ability to accurately model stochastic phenotypes may prove critical to understanding disease and therapeutic interventions in higher eukaryotes.
机译：与许多完全可行或无法生存的突变体不同，酿酒酵母的CLB2-dbDelta clb5Delta突变体在葡萄糖中不可行，但在较慢的培养基（如棉子糖）上则部分可行。在棉子糖上，突变细胞可以发芽并分裂，但在每个周期中，细胞都有可能分裂失败（末期停滞），从而使其退出细胞周期。这种影响导致无法通过确定性模型解释的随机表型。我们测量在棉子糖上生长的野生型和突变细胞的预算间时间，并计算统计数据和分布以表征突变体的行为。我们将出芽的酵母细胞周期的详细的确定性模型转换为随机模型，并确定其捕获突变菌株的随机表型的程度。数学模型的预测与我们的实验数据基本吻合，并为改进模型提供了指导。最终，对随机表型进行精确建模的能力可能被证明对于理解高等真核生物的疾病和治疗干预至关重要。

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