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首页> 外文期刊>Resuscitation. >A dose-response study of graded reoxygenation on the carotid haemodynamics, matrix metalloproteinase-2 activities and amino acid concentrations in the brain of asphyxiated newborn piglets.
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A dose-response study of graded reoxygenation on the carotid haemodynamics, matrix metalloproteinase-2 activities and amino acid concentrations in the brain of asphyxiated newborn piglets.

机译：分级复氧对窒息新生仔猪大脑中颈动脉血流动力学，基质金属蛋白酶2活性和氨基酸浓度的剂量反应研究。

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PURPOSE: It is controversial to choose an appropriate oxygen concentration to resuscitate asphyxiated newborns regarding the clinical and biochemical oxidative effects. We examined the vasomotor response to reoxygenation with graded reoxygenation and the effects on matrix metalloproteinases and amino acids of the immature brain. METHODS: Thirty-two piglets (1-3 days, 1.5-2.1 kg) were instrumented for continuous monitoring of left common carotid and pulmonary arterial flows (Transonic). Piglets were randomized to a sham-operated control group (without hypoxia/reoxygenation) or 2 h hypoxia induced by decreasing the inspired oxygen concentration to 10-15%, followed by reoxygenation with 21, 50 or 100% oxygen for 1 h and then 21% oxygen for 3 h (n=8 each). The brains were then flash frozen and analyzed for matrix metalloproteinases and amino acid levels by zymography and HPLC, respectively. RESULTS: After 2 h oxygen deprivation, the absolute carotid flow remained similar but accounted for 38% of cardiac output (increased from 17% at baseline, p=0.001). During early reoxygenation, the flow rose in the piglets resuscitated with air (p<0.05), but not in those with supplemental oxygen. Carotid vascular resistance correlated significantly with the arterial partial pressure of oxygen (r=0.7). There was an oxygen-dependent increase in global cerebral activity of matrix metalloproteinase-2 with specific increases in the basal ganglia of all hypoxic-reoxygenated brains. There were no significant differences in glutamate and other amino acids in any brain regions. CONCLUSIONS: Although using high oxygen concentration to resuscitate asphyxiated newborn piglets increased carotid vascular resistance and cerebral matrix metalloproteinase-2 activity, there is no detrimental effect observed in this acute model of hypoxia-reoxygenation.
机译：目的：就临床和生化氧化作用而言，选择合适的氧气浓度以复苏窒息新生儿是有争议的。我们检查了分级复氧对复氧的血管舒缩反应以及对未成熟大脑基质金属蛋白酶和氨基酸的影响。方法：对32只仔猪（1-3天，1.5-2.1kg）进行了监测，以连续监测左颈总动脉和肺动脉血流（Transonic）。将仔猪随机分为假手术对照组（无缺氧/复氧）或通过将吸入氧气浓度降低至10-15％诱导2小时缺氧，然后分别以21％，50％或100％氧气再充氧1小时，然后再21氧气百分比3小时（n = 8）。然后将大脑快速冷冻，并分别通过酶谱法和HPLC分析基质金属蛋白酶和氨基酸水平。结果：缺氧2小时后，绝对颈动脉血流量保持相似，但占心输出量的38％（从基线时的17％增加，p = 0.001）。在早期复氧期间，在用空气复苏的仔猪中流量增加（p <0.05），但在补充氧气的仔猪中没有增加。颈动脉血管阻力与氧气的动脉分压显着相关（r = 0.7）。基质金属蛋白酶2的整体脑活动具有氧依赖性增加，而所有低氧-复氧脑的基底神经节都有特定的增加。在任何大脑区域，谷氨酸和其他氨基酸没有显着差异。结论：尽管使用高氧浓度复苏窒息新生仔猪可增加颈动脉血管阻力和脑基质金属蛋白酶2活性，但在这种急性缺氧-复氧模型中未观察到有害作用。

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