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首页> 外文期刊>RSC Advances >Temporal changes in horsebean bioavailability and accumulation after removing extractable oxytetracycline fractions in soils
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Temporal changes in horsebean bioavailability and accumulation after removing extractable oxytetracycline fractions in soils

机译：去除土壤中可提取的土霉素成分后，大豆生物利用度和累积的时间变化

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摘要

Extractable fractions of oxytetracycline in soil affect its bioavailability and accumulation in plants. The objective was to assess the bioavailability of different bound fractions of oxytetracycline available in soil to horsebean. After freshly spiking with oxytetracycline for 24 h, soils were treated with water and mild (0.1 mol L-1 CaCl2) and exhaustive (0.1 mol L-1 Na(2)EDTA-McIlvaine) extractants as treatments T-1, T-2, and T-3, respectively, to obtain different extractable oxytetracycline fractions. The control was a nonextracted soil. Horsebean was exposed to the abovementioned soils. The results showed that oxytetracycline was accumulated in shoots and roots at 10 days; however, after 10 days, the amount of oxytetracycline in the roots was below its quantification limits (<0.001 mg kg(-1)). The remained OTC as resistant bound fraction in T-3 always exhibited the highest shoot concentration factors (SCF) compared with removing fewer extractable fractions during 14-28 days exposure. Moreover, the effect of horsebean upon oxytetracycline extractability during exposure was also studied. Adding horsebean did not affect the change trend of three extractable fractions in the control, T-2, or T-3 during exposure, but changed the trend of CaCl2- and total-extractable fractions in T-1. Horsebean activity can increase total-extractable concentration in the control, but decrease total-extractable concentration in T-1, T-2 and T-3. The correlation analysis showed that there were close relationships between water-, CaCl2-, total-extractable fractions in soil and root accumulation for the horsebean at 10 days of exposure. The interesting phenomenon was that resistant bound OTC had a high availability, and its ecological risk in soil had been underestimated in previous investigations(.)
机译：土壤中土霉素的可提取部分影响其生物利用度和在植物中的积累。目的是评估土壤中可利用的土霉素对大豆的不同结合部分的生物利用度。用土霉素新鲜加标24小时后，用水和温和的（0.1 mol L-1 CaCl2）和详尽的（0.1 mol L-1 Na（2）EDTA-McIlvaine）萃取剂处理土壤，作为处理剂T-1，T-2分别为T3和T-3，以获得不同的可提取的土霉素级分。对照是未提取的土壤。蚕豆暴露于上述土壤中。结果表明，土霉素在第10天根和茎中积累。但是，在10天后，根中的土霉素含量低于其定量限（<0.001 mg kg（-1））。与在暴露14-28天期间除去较少的可提取馏分相比，在T-3中作为抗性结合馏分保留的OTC始终显示出最高的芽浓度因子（SCF）。此外，还研究了蚕豆对暴露过程中土霉素提取能力的影响。添加蚕豆不会影响对照中T-2或T-3中三个可萃取级分在暴露过程中的变化趋势，但会改变CaCl2-和T-1中可提取总级分的趋势。蚕豆的活性可以增加对照中的总提取浓度，但降低T-1，T-2和T-3中的总提取浓度。相关分析表明，在暴露10天后，水分，CaCl2-，土壤中总可提取组分与大豆根系积累之间存在密切关系。有趣的现象是抗药性绑定的OTC具有很高的利用率，并且在以前的研究中低估了其在土壤中的生态风险。

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