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首页> 外文期刊>RSC Advances >Development of an improved E. coli bacterial strain for green and sustainable concrete technology
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Development of an improved E. coli bacterial strain for green and sustainable concrete technology

机译：开发用于绿色和可持续混凝土技术的改良大肠杆菌菌株

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Development of smart bioconcrete materials has recently become an emerging area of research for construction. Here, the silica leaching attribute transferred to an E. coli bacterial strain, has been utilized for higher strength and more durable concrete structures. The silica leaching gene was fished out from the BKH2 bacterium (GenBank accession no.: KP231522), amplified by the PCR technique and cloned into E. coli bacteria via a suitable T-vector to develop a bio-engineered E. coli strain. The transformed bacterial cells when incorporated directly into mortar specimens produced high performance biocomposite materials. Improvements on the compressive strength (>30%), ultrasonic pulse velocity (>5%), and decrease in the water absorption capacity were noted in the bacteria amended mortars. FESEM analysis revealed rod-like crystalline structures within the mortar matrices, and XRD analysis confirmed the development of a new silicate phase (gehlenite). The bioengineered E. coli cells can be directly explored for green and sustainable high performance composites in the near future.
机译：智能生物混凝土材料的开发最近已成为建筑研究的新兴领域。在此，已将二氧化硅浸出特性转移到大肠杆菌菌株中，用于更高的强度和更耐用的混凝土结构。从BKH2细菌（GenBank登录号：KP231522）中钓出二氧化硅浸出基因，通过PCR技术扩增，并通过合适的T-载体克隆到大肠杆菌中，以开发出生物工程的大肠杆菌菌株。将转化后的细菌细胞直接掺入研钵标本中时，可产生高性能的生物复合材料。在经细菌改良的砂浆中，抗压强度（> 30％），超声脉冲速度（> 5％）和吸水能力下降有所改善。 FESEM分析显示出灰浆基体中的棒状晶体结构，XRD分析证实了新的硅酸盐相（钠钙石）的形成。在不久的将来，可以直接探索生物工程化的大肠杆菌细胞，以获得绿色和可持续的高性能复合材料。

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