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Disposable dual sensor array for simultaneous determination of chlorogenic acid and caffeine from coffee

机译：一次性双传感器阵列，可同时测定咖啡中的绿原酸和咖啡因

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In this work a novel sensor array platform based on a dual carbon screen-printed electrode was developed for the simultaneous determination of chlorogenic acid and caffeine. One of the carbon working electrodes was modified with platinum nanoparticles, reduced graphene oxide and laccase (C-SPE/Pt-NPs/RGO/lacc-biosensor) for chlorogenic acid determination and the second carbon working electrodes was modified with reduced graphene oxide and Nafion (C-SPE/RGO/Nafion-sensor) for caffeine determination. Cyclic voltammetry was used to characterise and optimise the dual sensor array while chronoamperometry was used to investigate the bioelectrocatalytic response. The C-SPE/Pt-NPs/RGO/lacc for biosensing chlorogenic acid exhibited a sensitivity of 0.02 mu A mu M (1) and a detection limit of 2.67 mu M whereas the C-SPE/RGO/Nafion used for sensing caffeine has showed a sensitivity of 1.38 mu A mu M-1 and a detection limit of 0.22 mu M. The developed sensor array was used to determine these two major coffee bean compounds from real coffee samples. Due to its simplicity, feasibility and accessibility, the developed dual sensor array could represent the basis of a valuable analytical tool able to screen both chlorogenic acid and caffeine content from coffee samples offering important information about the phytochemical composition of the samples.
机译：在这项工作中，开发了一种基于双碳丝网印刷电极的新型传感器阵列平台，用于同时测定绿原酸和咖啡因。碳工作电极之一用铂纳米颗粒，还原的氧化石墨烯和漆酶（C-SPE / Pt-NPs / RGO / lacc-biosensor）修饰，用于绿原酸测定，第二个碳工作电极用还原的氧化石墨烯和Nafion修饰（C-SPE / RGO / Nafion-sensor）用于测定咖啡因。循环伏安法用于表征和优化双传感器阵列，而计时安培法用于研究生物电催化反应。用于生物检测绿原酸的C-SPE / Pt-NPs / RGO / lacc的灵敏度为0.02μAμM（1），检测限为2.67μM，而用于检测咖啡因的C-SPE / RGO / Nafion具有灵敏度为1.38μAμM-1，检测极限为0.22μM。开发的传感器阵列用于从真实咖啡样品中测定这两种主要的咖啡豆化合物。由于其简单性，可行性和可及性，开发的双传感器阵列可以代表有价值的分析工具的基础，该工具能够从咖啡样品中筛选绿原酸和咖啡因含量，从而提供有关样品植物化学成分的重要信息。

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