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首页> 外文期刊>RSC Advances >A biodegradable thermosensitive hydrogel with tuneable properties for mimicking three-dimensional microenvironments of stem cells
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A biodegradable thermosensitive hydrogel with tuneable properties for mimicking three-dimensional microenvironments of stem cells

机译：具有可调节特性的可生物降解的热敏水凝胶，可模拟干细胞的三维微环境

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Employing stem cells in therapeutic applications strongly depends on the extracellular three-dimensional (3D) microenvironment and cell carrier properties. In this work, chitosan-g-poly(N-isopropylacrylamide) (CS-g-PNIPAAm) was synthesized as a stem cell mimicking microenvironment. The influence of various polymerization conditions, such as acid concentration, reaction temperature and monomer feed, on the grafting parameters of this thermo-responsive hydrogel, was systematically investigated. We found that the resulting copolymers with a small amount of long poly(N-isopropylacrylamide) (PNIPAAm) side chains are low-soluble at low temperatures, but can form stronger hydrogels (almost 5 folds) at high temperatures, whereas copolymers with a high amount of short PNIPAAm side chains are more soluble at low temperatures, however, they cannot form strong hydrogels at high temperatures. In a physiological pH, an optimized balance between the solubility (as the pre-requirement for cell dispersion and injectability) of copolymers at ambient temperature and enhanced gel mechanical strength (as the essential parameter of stem cell microenvironments) at body temperature can be achieved through controlled reaction conditions. Mesenchymal stem cells (MSCs) were cultured in the CS-g-PNIPAAm hydrogels. Further analysis of confocal images confirms MSCs can maintain their viability and increase the cellular biomass inside hydrogels. Sectional analysis demonstrates that cells are uniformly distributed within the hydrogels. Our results confirm that the CS-g-PNIPAAm with manipulated properties could provide a potential 3D microenvironment for stem cell culture, differentiation and in vivo injection.
机译：在治疗应用中采用干细胞在很大程度上取决于细胞外三维（3D）微环境和细胞载体特性。在这项工作中，壳聚糖-g-聚（N-异丙基丙烯酰胺）（CS-g-PNIPAAm）被合成为模拟微环境的干细胞。系统地研究了各种聚合条件，例如酸浓度，反应温度和单体进料对这种热响应水凝胶的接枝参数的影响。我们发现所得的带有少量长聚（N-异丙基丙烯酰胺）（PNIPAAm）侧链的共聚物在低温下是低溶解性的，但在高温下却可以形成更强的水凝胶（几乎是5倍），而高分子量的共聚物一定数量的短PNIPAAm侧链在低温下更易溶解，但是在高温下它们不能形成坚固的水凝胶。在生理pH下，可以通过以下方法实现共聚物在环境温度下的溶解度（作为细胞分散性和注射性的先决条件）与增强的凝胶机械强度（作为干细胞微环境的基本参数）之间的最佳平衡。控制反应条件。间充质干细胞（MSCs）培养在CS-g-PNIPAAm水凝胶中。共聚焦图像的进一步分析证实，MSC可以维持其生存能力并增加水凝胶内部的细胞生物量。截面分析表明，细胞在水凝胶中均匀分布。我们的结果证实，具有可控特性的CS-g-PNIPAAm可以为干细胞培养，分化和体内注射提供潜在的3D微环境。

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