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A rapid immunomagnetic-bead-based immunoassay for triazophos analysis

机译：一种基于免疫磁珠的快速免疫分析法，用于三唑磷分析

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摘要

An immunomagnetic-bead-based enzyme-linked immunosorbent assay (IMB-ELISA) was developed for detection of pesticides by using carboxyl functionalized magnetic Fe3O4 nanoparticles (CMNPs). The CMNPs were prepared by co-precipitation of Fe2+/Fe3+ with oleic acid as a surfactant and subsequent oxidation of C=C into COOH by KMnO4 solution in situ. Then, anti-pesticide (triazophos) monoclonal antibodies were directly bonded onto the magnetic nanoparticles, which significantly increased the sensitivity compared with classic ELISA. The detection limit was 0.10 ng mL(-1). Addition-recovery and high-precision experiments were performed on blank samples that were determined to be without triazophos. The average recovery rate for three types of samples (with each spiking concentration measured 5 times in parallel) ranged from 83.1% to 115.9%, with a relative standard deviation (RSD) of less than 10%, which meets the requirement of pesticide residue analysis. The application results were in accordance with the gas chromatography-mass spectrometry (GC-MS) method, suggesting that IMB-ELISA is rapid and reliable for pesticide detection.
机译：通过使用羧基官能化的磁性Fe3O4纳米颗粒（CMNP），开发了一种基于免疫磁珠的酶联免疫吸附测定（IMB-ELISA）来检测农药。通过将Fe2 + / Fe3 +与油酸作为表面活性剂共沉淀，然后通过KMnO4原位将C = C氧化为COOH，制备CMNP。然后，将抗农药（triazophos）单克隆抗体直接结合到磁性纳米颗粒上，与经典ELISA相比，显着提高了灵敏度。检出限为0.10 ng mL（-1）。对确定不含三唑磷的空白样品进行了加法回收和高精度实验。三种样品（每次加标浓度平行测定5次）的平均回收率在83.1％至115.9％之间，相对标准偏差（RSD）小于10％，符合农药残留分析的要求。应用结果与气相色谱-质谱法（GC-MS）相吻合，表明IMB-ELISA快速，可靠地检测农药。

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