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首页> 外文期刊>Cellular Physiology and Biochemistry >Triggering of Suicidal Erythrocyte Death by Amphotericin B
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Triggering of Suicidal Erythrocyte Death by Amphotericin B

机译：两性霉素B触发自杀性红细胞死亡

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Amphotericin B is widely used as antifungal drug. Side effects include anemia. A variety of drugs and diseases associated with anemia has recently been shown to trigger suicidal erythrocyte death or eryptosis, i.e. cell membrane scrambling and cell shrinkage. Eryptosis may be triggered by increased cytosolic Ca2+ activity and by lack of ATP. The present study explored whether amphotericin B stimulates eryptosis. Cell membrane scrambling was estimated from annexin V-binding to phosphatidylserine exposed at the cell surface, cell shrinkage from forward scatter in FACS analysis, cytosolic Ca2+ activity from Fluo3 fluorescence and the cytosolic ATP concentration from a luciferase-based assay. Exposure to amphotericin B (0.1-1 mu g/ml) within 48 hours significantly increased annexin V-binding, decreased forward scatter, increased cytosolic Ca2+ activity and decreased cytosolic ATP content. In conclusion, amphotericin B stimulates suicidal cell death of erythrocytes, which may in turn contribute to the clearance of circulating erythrocytes and thus to anemia.
机译：两性霉素B被广泛用作抗真菌药。副作用包括贫血。最近已显示出与贫血有关的多种药物和疾病可引起自杀性红细胞死亡或隐匿，即细胞膜争夺和细胞收缩。胞质Ca2 +活性增加和ATP缺乏可能触发加密。本研究探讨了两性霉素B是否刺激隐匿性。根据膜联蛋白V与暴露于细胞表面的磷脂酰丝氨酸的结合，FACS分析中向前散射引起的细胞收缩，Fluo3荧光引起的胞质Ca2 +活性以及基于萤光素酶测定的胞质ATP浓度来估计细胞膜的混乱。在48小时内暴露于两性霉素B（0.1-1μg/ ml）会显着增加膜联蛋白V结合，降低前向散射，增加胞质Ca2 +活性并降低胞质ATP含量。总之，两性霉素B刺激了红细胞的自杀性细胞死亡，这可能反过来有助于循环红细胞的清除，从而导致贫血。

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