• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Qualitative and quantitative analyses of goitrin-epigoitrin in Isatis indigotica using supercritical fluid chromatography-photodiode array detector-mass spectrometry
【24h】

Qualitative and quantitative analyses of goitrin-epigoitrin in Isatis indigotica using supercritical fluid chromatography-photodiode array detector-mass spectrometry

机译：超临界流体色谱-光电二极管阵列检测器-质谱法定性分析板蓝根中鹅甲状腺素-鬼臼素的定性和定量分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel comprehensive method using supercritical fluid chromatography-photodiode array detector-mass spectrometry (SFC-PDA-MS) was developed for the qualitative and quantitative analyses of a chiral isomer pair in Ban Lan Gen (dried root of Isatis indigotica) and the processed product. A standard goitrin-epigoitrin racemic mixture was used for method development. A total of six chiral stationary phases (CSPs) were screened and the (S,S)-Whelk-O 1 (4.6 x 250 mm, 10 mu m) column was chosen as it offers baseline resolution for the enantiomeric pair with separation accomplished in 6 minutes. A single quadrupole MS and a PDA detector were used in line for the detection. The validated method was applied successfully in the analysis of different samples. Results indicated that the developed assay was fast, sensitive and reproducible. SFC should be an integral part of the overall analytical platform for TCM and natural product research, especially in the area of chiral analysis.
机译：建立了一种超临界流体色谱-光电二极管阵列检测器-质谱（SFC-PDA-MS）的综合方法，用于对板蓝根（蓝靛蓝的干燥根）中的手性异构体对及其加工产物进行定性和定量分析。 。使用标准的甲状腺素-表鬼臼素外消旋混合物进行方法开发。总共筛选了六个手性固定相（CSP），并选择了（S，S）-Whelk-O 1（4.6 x 250 mm，10μm）色谱柱，因为它为对映异构体对提供了基线分离度，并完成了分离6分钟在线使用单个四极杆MS和PDA检测器进行检测。经验证的方法已成功应用于不同样品的分析。结果表明，开发的检测方法快速，灵敏且可重复。 SFC应该是中药和天然产物研究的整体分析平台的组成部分，尤其是在手性分析领域。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号