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Preparation and evaluation of a novel pADM-derived micro- and nano electrospun collagen membrane

机译：新型pADM衍生的微纳纳米电纺胶原膜的制备与评价

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As the three-dimensional (3D) architecture of porcine acellular dermal matrix (pADM) mimics the biological characteristics of a native extracellular matrix (ECM), it has been extensively utilized as a tissue scaffold. In this study, a novel pADM-derived micro-and nano electrospun collagen membrane (PDEC) was successfully prepared by the electrospinning technique, using porcine acellular dermal matrix (pADM) as the raw material and 1,1,1,3,3,3-hexafluoro-2-propanol (HIFP) as the solvent, and was characterized by scanning electron microscopy (SEM) analysis. In contrast, another collagen-derived electrospun collagen (CDEC) was also fabricated using pure porcine collagen as the raw material. The structure and composition of the PDEC and CDEC were measured by Fourier transform infrared (FTIR) spectroscopy, SDS-PAGE gel electrophoresis, specific intrinsic viscosity and atomic force microscopy (AFM). The results indicate that the structural integrity of the PDEC is almost maintained and only a small amount of the PDEC was destroyed into gelatin, while almost all of the CDEC was degraded. Moreover, the results of circular dichroism (CD) analysis also demonstrate that the PDEC possesses a higher content of a-helix structure but less beta-turn structure. Additionally, the results from ultrasensitive differential scanning calorimetry (US-DSC) and XRD analysis also suggest that the thermal stability and crystallization of the PDEC have little differences compared to collagen. Furthermore, the mechanical properties of the PDEC are significantly enhanced compared to those of the CDEC. Above all, the results obtained in the MTT study indicate that the PDEC has almost the same good biological activity, in terms of cytotoxicity, as natural porcine collagen, and this activity is obviously superior to that of the CDEC. In conclusion, our study provides a new pADM-derived electrospun collagen in which the triple helical structure has seldom been damaged and thus it can be applied as a novel electrospun collagen scaffold for tissue engineering.
机译：由于猪脱细胞真皮基质（pADM）的三维（3D）结构模仿了天然细胞外基质（ECM）的生物学特性，因此已被广泛用作组织支架。在这项研究中，以猪脱细胞真皮基质（pADM）为原料，并以1,1,1,3,3,1,1,1,1,3,3为原料，通过静电纺丝技术成功制备了一种新的pADM衍生的微纳米电纺胶原膜（PDEC）。以3-六氟-2-丙醇（HIFP）为溶剂，并通过扫描电子显微镜（SEM）分析进行了表征。相反，还使用纯猪胶原蛋白为原料制备了另一种胶原蛋白衍生的静电纺丝胶原蛋白（CDEC）。 PDEC和CDEC的结构和组成通过傅立叶变换红外（FTIR）光谱，SDS-PAGE凝胶电泳，比重特性粘度和原子力显微镜（AFM）进行测量。结果表明，几乎保持了PDEC的结构完整性，只有少量PDEC被破坏成明胶，而几乎所有的CDEC都被降解了。此外，圆二色性（CD）分析的结果还表明，PDEC具有较高含量的a-螺旋结构，但具有较少的β-转角结构。此外，超灵敏差示扫描量热法（US-DSC）和XRD分析的结果还表明，与胶原蛋白相比，PDEC的热稳定性和结晶度差异很小。此外，与CDEC相比，PDEC的机械性能得到了显着提高。最重要的是，在MTT研究中获得的结果表明，就细胞毒性而言，PDEC具有与天然猪胶原几乎相同的良好生物学活性，并且该活性明显优于CDEC。总之，我们的研究提供了一种新的pADM衍生的电纺胶原蛋白，其中三螺旋结构很少被破坏，因此可以用作组织工程的新型电纺胶原蛋白支架。

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