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首页> 外文期刊>Russian Journal of Plant Physiology >Effect of spermine treatment on the functioning of Thellungiella salsuginea antioxidant system.
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Effect of spermine treatment on the functioning of Thellungiella salsuginea antioxidant system.

机译：精胺处理对Salsungineella抗氧化系统功能的影响。

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The effect of spermine (Spm) treatment on the content of polyamines (PAs) and activities of antioxidant enzymes in the roots and leaves of Thellungiella salsuginea (Pall.) O.E. Schulz plants grown under optimal conditions were studied. The genes encoding three forms of ascorbate peroxidase (APX; APX1, APX2, and APX4) and genes of key enzymes of proline metabolism (Pro, P5CS1, 1P5CD) were identified, and their expression intensity was measured. Six-day-old plants were treated with Spm (1 and 2 mM) and with the inhibitor of polyamine oxidase (PAO) activity, N,N-(2-hydroxyethyl)hydrazine (HEH, 1 and 2 mM) separately or in combination by adding these compounds to nutrient medium. Roots and leaves responded differently to Spm treatment. In the leaves, the content of PAs reduced due to a decreased in the spermidine (Spd) content, whereas in the roots the total pool of PAs increased due to putrescine (Put) and Spd accumulation. Treatment with Spm activated PAO in the roots but not in the leaves; HEH removed this increase, but the intercellular Spm concentration was not substantially changed. It was suggested that treatment with Spm suppressed the biosynthesis of intracellular Spm and, on the other hand, stimulated the reverse conversion of Spm into Spd and further into Put due to the activation of one of the PAO isoforms. Plant treatment with Spm was not accompanied by a noticeable activation enzymes degrading hydrogen peroxide in the roots: APX, (except of peroxidase II), and catalase. However, the activity of Cu/Zn-SOD doubled and the activity of Mn-SOD reduced. In the leaves, slight activation of peroxidases I and III, the inhibition of Cu/Zn- and Mn-SOD, differential changes in the time-coursed of gene expression of three APX isoforms, and activated gene expression of key enzymes of Pro metabolism were observed. At the same time, the level of MDA did not increase either in the leaves or in the roots. This indicates that treatment of Th. salsuginea plants with Spm under optimal growing conditions did not enhance ROS generation and did not manifest prooxidant properties.Notes Translated from Fiziologiya Rastenii (2014) 61 (1), 69-76 (Ru).
机译：精胺（Spm）处理对Salsuginea（Pall。）O.E.根和叶中多胺（PAs）含量和抗氧化酶活性的影响。研究了在最佳条件下生长的舒尔茨植物。确定了编码三种形式的抗坏血酸过氧化物酶（APX; APX1，APX2和APX4）的基因和脯氨酸代谢关键酶的基因（Pro，P5CS1、1P5CD），并测量了它们的表达强度。六天大的植物分别用Spm（1和2 mM）和多胺氧化酶（PAO）活性抑制剂，N，N-（2-羟乙基）肼（HEH，1和2 mM）处理或组合处理通过将这些化合物添加到营养培养基中。根和叶对Spm处理的反应不同。在叶片中，PA的含量由于亚精胺（Spd）含量的减少而降低，而在根部，PA的总库因腐胺（Put）和Spd的积累而增加。用SPm处理可在根部而不是叶中激活PAO; HEH消除了这种增加，但细胞间Spm浓度没有实质性改变。有人提出，用Spm处理可抑制细胞内Spm的生物合成，另一方面，由于一种PAO同工型的激活，刺激了Spm逆转化为Spd并进一步转化为Put。用Spm处理植物并没有伴随着明显的激活酶降解根部的过氧化氢：APX（过氧化物酶II除外）和过氧化氢酶。但是，Cu / Zn-SOD的活性增加了一倍，Mn-SOD的活性降低。在叶片中，过氧化物酶I和III的轻微活化，对Cu / Zn-和Mn-SOD的抑制，三种APX亚型的基因表达随时间变化的差异以及Pro代谢关键酶的活化基因表达为：观测到的。同时，叶片或根部的MDA水平均未增加。这表明Th的治疗。在最佳生长条件下具有Spm的Salsuginea植物不会增强ROS的产生，也没有表现出抗氧化特性。注释译自Fiziologiya Rastenii（2014）61（1），69-76（Ru）。

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