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首页> 外文期刊>Cellular Signalling >Differentiation inducing factor-1 (DIF-1) induces gene and protein expression of the Dictyrostelium nuclear calmodulin-binding protein nucleomorphin
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Differentiation inducing factor-1 (DIF-1) induces gene and protein expression of the Dictyrostelium nuclear calmodulin-binding protein nucleomorphin

机译：分化诱导因子-1（DIF-1）诱导双歧杆菌核钙调蛋白结合蛋白核吗啡肽的基因和蛋白表达

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摘要

The nucleomorphin gene numA1 from Dicryostelium codes for a multi-domain, calmodulin binding protein that regulates nuclear number. To gain insight into the regulation of numA, we assessed the effects of the stalk cell differentiation inducing factor-1 (DIF-1), an extracellular signalling molecule, on the expression of numA1 RNA and protein. For comparison, the extracellular signalling molecules cAMP (mediates chemotaxis, prestalk and prespore differentiation) and ammonia (NH3/NH4+; antagonizes DIF) were also studied. Starvation, which is a signal for multicellular development, results in a greater than 80% decrease in numA I mRNA expression within 4 h. Treatment with ammonium chloride led to a greater than 90% inhibition of numA1 RNA expression within 2 h. In contrast, the addition of DIF-1 completely blocked the decrease in numA1 gene expression caused by starvation. Treatment of vegetative cells with cAMP led to decreases in numA1 RNA expression that were equivalent to those seen with starvation. Western blotting after various morphogen treatments showed that the maintenance of vegetative levels of numA1 RNA by DIF-1 in starved cells was reflected in significantly increased numA1 protein levels. Treatment with cAMP and/or ammonia led to decreased protein expression and each of these morphogens suppressed the stimulatory effects of DIF-1. Protein expression levels of CBP4a, a calcium-dependent binding partner of numA1, were regulated in the same manner as numA1 suggesting this potential co-regulation may be related to their functional relationship. NumA1 is the first calmodulin binding protein shown to be regulated by developmental morphogens in Dictyostelium being upregulated by DIF-1 and down-regulated by cAMP and ammonia. (c) 2008 Elsevier Inc. All rights reserved.
机译：Dicryostelium中的吗啡基因numA1编码多域钙调蛋白结合蛋白，可调节核数。为了深入了解numA的调控，我们评估了茎细胞分化诱导因子1（DIF-1）（一种细胞外信号分子）对numA1 RNA和蛋白质表达的影响。为了进行比较，还研究了细胞外信号分子cAMP（介导趋化性，茎前和孢子前分化）和氨（NH3 / NH4 +;拮抗DIF）。饥饿是多细胞发育的信号，导致numA I mRNA表达在4小时内下降超过80％。用氯化铵处理导致2小时内对numA1 RNA表达的抑制作用大于90％。相反，DIF-1的添加完全阻止了饥饿引起的numA1基因表达的下降。用cAMP处理营养细胞导致numA1 RNA表达下降，这与饥饿时的情况相同。各种形态发生素处理后的蛋白质印迹显示，饥饿细胞中DIF-1对numA1 RNA营养水平的维持反映为numA1蛋白水平显着增加。用cAMP和/或氨水处理会导致蛋白质表达下降，并且这些形态原均抑制DIF-1的刺激作用。以与numA1相同的方式调节CBP4a（numA1的钙依赖性结合伴侣）的蛋白表达水平，表明该潜在的共调节可能与其功能关系有关。 NumA1是第一个钙调蛋白结合蛋白，显示出受网柄菌属中的发育形态发生子调节，并被DIF-1上调，而cAMP和氨气下调。 （c）2008 Elsevier Inc.保留所有权利。

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