• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Oncology letters >Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
【24h】

Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

机译：金莲花总黄酮对人乳腺癌MCF-7细胞的生长抑制和凋亡作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991-1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-kappa B expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.
机译：金莲花的干花长期以来一直被用作重要的中药。先前的研究已证明中华三叶黄酮具有减少人乳腺癌MCF-7细胞增殖的能力。本研究进一步研究了中华三叶黄酮对MCF-7细胞生长和增殖的影响，并在0.0991-1.5856 mg / ml的浓度范围内观察到明显的抑制作用。通过微分干涉对比软件，Hoechst 33258方法，扫描电子显微镜，苏木精/曙红染色和激光共聚焦显微镜评估了中华三叶黄酮处理引发的细胞凋亡。用中华三叶黄酮处理的细胞选择性降低bcl-2和NF-κB的表达，并增加caspase-9和caspase-3的表达，这表明细胞增殖的抑制是通过线粒体途径的激活而发生的。两者合计，结果证实了中华三叶黄酮抑制细胞增殖的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号