• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biological chemistry >Molecular Cloning and Characterization of a Novel Tissue-Specific Calpain Predominantly Expressed in the Digestive Tract
【24h】

Molecular Cloning and Characterization of a Novel Tissue-Specific Calpain Predominantly Expressed in the Digestive Tract

机译：主要在消化道中表达的新型组织特异性钙蛋白酶的分子克隆和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the course of the genomic cloning of nCL-2, a stomach-specific calpain, we identified a genomic clone encoding a novel member of the calpain large subunit family and designated it 'nCL-4'. First, using exon sequences, we cloned the cDNA for mouse nCL-4. Based on this sequence we also cloned the cDNAs for rat and human nCL-4. In the case of human nCL-4, the longest open reading frame encodes 690 amino acid residues (M_r 79095) with equal sequence similarities (50-55%) to both ubiquitous and organ-specific calpain large subunits from mammals. The deduced amino acid sequence revealed that nCL-4 is highly conserved among mammals. nCL-4 can be aligned without significant deletions or insertions, and, thus, like other calpains, can be divided into four domains (I-IV). The significant similarity of domains II and IV to those in conventional calpain large subunits suggests the potential protease activity and Ca~(2+) -binding ability of nCL-4. Northern blot analysis revealed that the mRNA for nCL-4 is expressed predominantly in stomach and small intestine but not in uterus, suggesting specialized functions of nCL-4 in the digestive tract. When overexpressed in COS-7 cells, a specific band for nCL-4 was detected. In addition, the gene coding for nCL-4 was localized on human chromosome 1.
机译：在nCL-2（一种胃特异的钙蛋白酶）的基因组克隆过程中，我们鉴定了一个编码calpain大亚基家族新成员的基因组克隆，并将其命名为“ nCL-4”。首先，我们使用外显子序列，克隆了小鼠nCL-4的cDNA。基于此序列，我们还克隆了大鼠和人nCL-4的cDNA。就人类nCL-4而言，最长的开放阅读框编码690个氨基酸残基（M_r 79095），与来自哺乳动物的普遍存在的和器官特异性的钙蛋白酶大亚基具有相同的序列相似性（50-55％）。推导的氨基酸序列揭示了nCL-4在哺乳动物中是高度保守的。 nCL-4可以比对而没有明显的缺失或插入，因此，与其他钙蛋白酶一样，它可以分为四个域（I-IV）。结构域II和IV与常规钙蛋白酶大亚基的结构域显着相似，表明nCL-4具有潜在的蛋白酶活性和Ca〜（2+）结合能力。 Northern印迹分析表明，nCL-4的mRNA主要在胃和小肠中表达，而在子宫中不表达，表明nCL-4在消化道中具有专门的功能。当在COS-7细胞中过表达时，检测到nCL-4的特定条带。此外，编码nCL-4的基因位于人类1号染色体上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号