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首页> 外文期刊>Oncology reports >Crude saponins from Platycodon grandiflorum induce apoptotic cell death in RC-58T/h/SA#4 prostate cancer cells through the activation of caspase cascades and apoptosis-inducing factor
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Crude saponins from Platycodon grandiflorum induce apoptotic cell death in RC-58T/h/SA#4 prostate cancer cells through the activation of caspase cascades and apoptosis-inducing factor

机译：桔梗粗制皂苷通过半胱天冬酶级联反应和凋亡诱导因子的活化，诱导RC-58T / h / SA＃4前列腺癌细胞凋亡。

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摘要

Saponins are a major active component of Platycodon grandiflorum (P. grandiflorum) and are known to induce apoptosis in metastatic prostate cancer cell lines. However, thus far, no research has been conducted on the anticancer activity of saponins in RC-58T/h/SA#4 primary prostate cancer cells. In this study, we show that the treatment of prostate cancer cells with saponins extracted from P. grandiflorum (SPG) inhibits cell proliferation in a dose-dependent manner. SPG significantly induced apoptotic cell death, resulting in an increase in the sub-G1 apoptotic cell population, apoptotic DNA fragmentation and morphological changes. Pre-treatment with a caspase inhibitor modestly attenuated the SPG-induced increase in the sub-G1 cell population, suggesting that caspases play a role in SPG-induced apoptosis. Moreover, SPG-induced apoptosis was associated with changes in caspase activity, the upregulation of the apoptotic protein, Bax and the downregulation of the anti-apoptotic protein, Bcl-2. Furthermore, the caspase-independent mitochondrial apoptosis factor, apoptosis-inducing factor (AIF) was upregulated following SPG treatment. These findings indicate that SPG exerts its anticancer effects on RC-58T/h/SA#4 primary prostate cancer cells through mitochondrial caspase-dependent and -independent apoptotic pathways.
机译：皂苷是桔梗（P. grandiflorum）的主要活性成分，已知可在转移性前列腺癌细胞系中诱导凋亡。然而，迄今为止，尚未对皂苷在RC-58T / h / SA＃4原发性前列腺癌细胞中的抗癌活性进行研究。在这项研究中，我们表明，用从大花假单胞菌（SPG）中提取的皂苷治疗前列腺癌细胞会以剂量依赖的方式抑制细胞增殖。 SPG显着诱导凋亡细胞死亡，从而导致亚G1凋亡细胞数量增加，凋亡DNA片段化和形态变化。用半胱天冬酶抑制剂进行的预处理可适度减弱SPG诱导的sub-G1细胞群体的增加，这表明半胱氨酸蛋白酶在SPG诱导的细胞凋亡中起作用。此外，SPG诱导的凋亡与caspase活性的变化，凋亡蛋白Bax的上调和抗凋亡蛋白Bcl-2的下调有关。此外，SPG处理后，不依赖caspase的线粒体凋亡因子，凋亡诱导因子（AIF）上调。这些发现表明SPG通过线粒体半胱天冬酶依赖性和非依赖性凋亡途径对RC-58T / h / SA＃4原发性前列腺癌细胞发挥抗癌作用。

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