• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Obesity research >A survey of genes differentially expressed in subcutaneous and visceral adipose tissue in men.
【24h】

A survey of genes differentially expressed in subcutaneous and visceral adipose tissue in men.

机译：男性皮下和内脏脂肪组织中差异表达基因的调查。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adipose tissue located within the abdominal cavity has been suggested to be functionally and metabolically distinct from that of the subcutaneous compartment. These differences could play a role in obesity-related complications. The aim of this study was to compare gene expression profiles of subcutaneous and visceral adipose tissues of 10 nondiabetic, normolipidemic obese men. Affymetrix human U133A arrays (10 arrays for subcutaneous fat samples and 10 arrays for visceral fat samples) were used. Differential gene expression was confirmed by real-time polymerase chain reaction in a subset of genes. A total of 5894 transcripts were detected in both depots in all 10 subjects, and 409 transcripts representing 347 encoded genes were differentially expressed. Of these, 131 genes were expressed at higher levels in subcutaneous adipose tissue, and 216 were expressed more abundantly in visceral fat. Differentially expressed profiles included genes of the Wnt signaling pathway, as well as CEPBA and HOX genes. In addition, genes involved in lipolytic stimuli and cytokine secretion were differentially expressed. The identification of a consistent and rather uniform pattern of differentially expressed genes between the two fat depots using multiple array replicates (10 arrays per fat compartment) generated new perspectives for future research on regional differences in adipose tissue biology.
机译：已经提出位于腹腔内的脂肪组织在功能和代谢上与皮下隔室不同。这些差异可能在肥胖相关并发症中起作用。这项研究的目的是比较10位非糖尿病，高血脂肥胖男性的皮下和内脏脂肪组织的基因表达谱。使用Affymetrix人U133A阵列（用于皮下脂肪样品的10个阵列和用于内脏脂肪样品的10个阵列）。通过实时聚合酶链反应在一个基因子集中证实了差异基因的表达。在所有10个受试者的两个库中总共检测到5894个转录本，代表347个编码基因的409个转录本被差异表达。其中，131个基因在皮下脂肪组织中高水平表达，216个在内脏脂肪中表达更高。差异表达的概况包括Wnt信号通路的基因，以及CEPBA和HOX基因。另外，参与脂解刺激和细胞因子分泌的基因被差异表达。使用多个阵列复制品（每个脂肪区隔10个阵列）来鉴定两个脂肪库之间差异表达基因的一致且相当均匀的模式，这为未来研究脂肪组织生物学的区域差异提供了新的观点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号