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Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

机译:同时时间和波长分辨荧光光谱用于近实时组织诊断

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摘要

A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems. (C) 2008 Optical Society of America.
机译:演示了一种新颖的基于光纤的方法,用于同时诊断时间和波长,用于快速诊断病变组织。通过将多个带通滤光片和二向色滤光片(405 / 40、460 / 50和550/50)与不同长度的光纤(1、10和19 m)用作光延迟,该系统可以实现近实时采集使用单个检测器和激发输入对时间分辨的荧光光谱进行表征和表征。选定波长处的多个荧光响应脉冲的记录可以在数百纳秒内完成,这提供了生物系统实时表征的功能。 (C)2008年美国眼镜学会。

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