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Flow imaging by use of femtosecond-laser-induced two-photon fluorescence

机译:飞秒激光诱导的双光子荧光成像

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摘要

A novel technique is demonstrated for the imaging of turbulent flows in which a single window to the flow is the only optical access required. A femtosecond laser is used to excite two-photon fluorescence in a disodium-fluorescein-seeded water jet. The fluorescence signal is generated at only the focal point of the laser because of the highly nonlinear nature of the two-photon absorption, and it is collected in a direction counterpropagating to the excitation beam. Tight focusing of the laser is used to limit the probe volume, and the two-dimensional mean and rms concentration images are collected by raster scanning the laser.
机译:展示了一种用于湍流成像的新颖技术,其中,唯一的光学通道是通向气流的单个窗口。飞秒激光用于在接种二钠荧光素的水射流中激发双光子荧光。由于双光子吸收的高度非线性特性,荧光信号仅在激光器的焦点处产生,并且沿与激发光束相反的方向收集。使用激光的严格聚焦来限制探头的体积,并通过光栅扫描激光来收集二维平均和均方根浓度图像。

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