...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Scientia horticulturae >Transcriptome analysis of fruit development of a citrus late-ripening mutant by microarray
【24h】

Transcriptome analysis of fruit development of a citrus late-ripening mutant by microarray

机译：柑橘后期晚熟突变体果实发育的转录组分析

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mingliutianju (Citrus reticuiata Blanco cv.Mingliutianju, MP) is a novel late ripening cultivar selected from Chuntianju (Citrus reticuiata Blanco cv.Chuntianju, CP) through spontaneous bud mutation. Large-scale analysis of gene expression differences between of peels of MP and CP is studied during the course of 12 weeks and 23 weeks after flowering (WAF) using Affymetrix GeneChip (R) citrus genome array to provide new insights into the mechanisms behind this bud mutation. Altogether, 395 differentially expressed genes are identified including 132 up-regulated and 263 down-regulated genes. Singular enrichment analysis (SEA) and parametric analysis of gene set enrichment (PAGE) are performed and 7 statistically significant differentially expressed genes are selected finally for further study. Results from semiquantitative RT-PCR performed on these 7 genes show that one gene's expression situation is totally correlated with that observed with the microarray. These seven genes include three UDP-glucosyltransferase genes, two disease resistance-responsive protein-related genes, one chitinase gene and one protein kinase family protein gene, all of which are involved in 18 different biological processes according to the hierarchical tree of annotated gene ontology (GO) terms. The candidate biological process and its relation to bud mutation are discussed. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
机译：明柳田菊（Citrus reticuiata Blanco cv.Mingliutianju，MP）是通过自然芽突变从春田菊（Citrus reticuiata Blanco cv.Chuntianju，CP）选育的新型晚熟品种。使用Affymetrix GeneChip（R）柑橘基因组阵列对开花后（WAF）的12周和23周过程中的MP和CP果皮之间的基因表达差异进行大规模分析，以提供对该芽背后机制的新见解突变。总共鉴定出395个差异表达基因，包括132个上调基因和263个下调基因。进行了奇异富集分析（SEA）和基因组富集的参数分析（PAGE），最后选择了7个具有统计学意义的差异表达基因进行进一步研究。对这7个基因进行的半定量RT-PCR结果表明，一个基因的表达情况与微阵列观察到的情况完全相关。这七个基因包括三个UDP-葡萄糖基转移酶基因，两个抗病反应蛋白相关基因，一个几丁质酶基因和一个蛋白激酶家族蛋白基因，根据带注释的基因本体的层次结构树，它们全部涉及18个不同的生物学过程。 （GO）条款。讨论了候选生物过程及其与芽突变的关系。 Crown版权所有（C）2011，由Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号