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首页> 外文期刊>Separation and Purification Technology >Supercritical carbon dioxide anti-solvent precipitation of anti-oxidative zeaxanthin highly recovered by elution chromatography from Nannochloropsis oculata
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Supercritical carbon dioxide anti-solvent precipitation of anti-oxidative zeaxanthin highly recovered by elution chromatography from Nannochloropsis oculata

机译：超临界二氧化碳反溶剂沉淀法从南绿藻中洗脱色谱法高回收率的抗氧化玉米黄质

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摘要

Zeaxanthin, a kind of xanthophylls, rich in Marigold flowers and a few micro algae, acts as a strong antioxidant capable of protecting human eye-sight from age-related macular degeneration. This study examined elution chromatography coupled with supercritical anti-solvent precipitation applied for generation of zeaxanthin-rich particulates from micro-algal Nannochloropsis oculata species. Crude of the ultrasonic acetone extract subjected to the column fractionation successfully yields a fraction containing 303.02 mg/g (30%) of zeaxanthin with recovery of 90%. Supercritical anti-solvent precipitation of the algal solution at 200 bar and 313 K was used in producing submicron-sized particulates of 50% zeaxanthin. These extracts were subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate free-radical assay and human adult retinal pigment epithelium-19 cells. This work provided a fast and environmental benign process to generate anti-oxidative capable algal micronized powders from the N. oculata.
机译：玉米黄质，一种叶黄素，富含万寿菊花和少量微藻，是一种强抗氧化剂，能够保护人的视力免受与年龄有关的黄斑变性。这项研究检查了洗脱色谱结合超临界抗溶剂沉淀技术，该沉淀技术可用于从微藻Nannochloropsis oculata物种产生富含玉米黄素的颗粒。进行柱分离的超声丙酮提取物粗制物成功产生了含有303.02 mg / g（30％）玉米黄质的馏分，回收率为90％。在200 bar和313 K的压力下，藻类溶液的超临界反溶剂沉淀法可用于生产50％玉米黄质的亚微米级颗粒。这些提取物在2，2-二苯基-2-吡啶并肼水合物自由基测定法和人成年视网膜色素上皮-19细胞中进行抗氧化能力的测试。这项工作提供了一种快速，环境友好的方法，可以从N. oculata产生抗氧化的藻类微粉化粉末。

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