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首页> 外文期刊>Separation and Purification Technology >Vinyl-2-pyrrolidone derivatized guar gum based aqueous two-phase system
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Vinyl-2-pyrrolidone derivatized guar gum based aqueous two-phase system

机译：乙烯基-2-吡咯烷酮衍生的瓜尔胶基水性两相体系

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摘要

Aqueous two-phase systems (ATPs) are formed when certain combinations of two polymers or polymer and salt are added to water. Phase separation occurs at certain specific concentrations that have similar densities and low interfacial tensions. The present study involves synthesis and application of a novel derivatized guar in aqueous two-phase system. The derivation of guar gum was performed by grafting of 1 -vinyl-2-pyrrolidone by a redox reaction in aqueous medium ceric ammonium nitrate (CAN) and potassium peroxydisulfate (K2S2O8). The ratio of grafting efficiency (%GE) and ratio of grafting (%G) was 56% and 70.1 % respectively. The grafted copolymers were characterized for percent yield and FTIR absorbance. A new aqueous two-phase extraction protocol was established using a mixture of derivatized guar and dextran. A phase diagram was constructed based on the phase separation exhibited by the system at 25 °C. The partition coefficient was determined for standard bovine serum albumin (BSA), lactate oxidase (LO) and phytase. The partitioning coefficients were 0.479,1.981, and 2.586 respectively in the Dextran (2 million)/V-GG/H2O system. The influence of an ionic salt sodium chloride (NaCl) was investigated on the partitioning of BSA.
机译：当将两种聚合物或聚合物和盐的某些组合添加到水中时，就会形成两相水体系（ATP）。在具有类似密度和低界面张力的某些特定浓度下发生相分离。本研究涉及水两相体系中新型衍生瓜尔胶的合成与应用。瓜耳胶的衍生是通过在硝酸铈铵（CAN）和过氧二硫酸钾（K2S2O8）的水性介质中通过氧化还原反应接枝1-乙烯基-2-吡咯烷酮来进行的。接枝率（％GE）和接枝率（％G）分别为56％和70.1％。表征了接枝共聚物的百分收率和FTIR吸光度。使用衍生的瓜尔胶和右旋糖酐的混合物建立了一种新的两相水相提取方案。根据系统在25°C下显示的相分离构造相图。确定标准牛血清白蛋白（BSA），乳酸氧化酶（LO）和植酸酶的分配系数。在Dextran（200万）/ V-GG / H2O系统中，分配系数分别为0.479、1.981和2.586。研究了离子盐氯化钠（NaCl）对BSA分配的影响。

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