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Isolation of lactoferrin from acid whey by magnetic affinity separation

机译：磁性亲和分离法从酸性乳清中分离乳铁蛋白

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摘要

A micron-sized monodisperse superparamagnetic polyglycidyl methacrylate (PGMA) particles coupled with heparin (PGMA-heparin) was synthesized by the polymerization, and then was evaluated as a method for purifying lactoferrin (LF) from bovine whey. The content of heparin groups at the surface of PGMA particles was 0.92 mg/g. Magnetic PGMA-heparin particles were highly effective in recovering LF directly from acid whey by a single step, whose purity was higher than that of commercial standard proteins, using NaCl solutions as eluent. The maximum LF binding capacity was 164 mg/g. The competitive adsorption tests verified that the magnetic approach offered a fast process and potential at a large scale. The practical use of magnetic PGMA-heparin particles for production of high purified LF from whey is a realistic prospect.
机译：通过聚合反应合成了与肝素偶联的微米级单分散超顺磁性聚甲基丙烯酸缩水甘油酯（PGMA）颗粒（PGMA-肝素），然后作为从牛乳清中纯化乳铁蛋白（LF）的方法进行了评估。 PGMA颗粒表面的肝素基含量为0.92mg / g。使用NaCl溶液作为洗脱剂，磁性PGMA-肝素颗粒可高效一步一步直接从酸乳清中回收LF，其纯度高于商业标准蛋白。 LF的最大结合能力为164 mg / g。竞争性吸附测试证明，磁性方法可提供快速的过程和大规模的潜力。磁性PGMA-肝素颗粒用于从乳清生产高纯度LF的实际应用是一个现实的前景。

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