Spectrophotometric determination of DNA using a near infrared probe 1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine

Wang H; Li WR; Lu Y; Fu NN; Zhang HS

首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Spectrophotometric determination of DNA using a near infrared probe 1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine

【24h】

Spectrophotometric determination of DNA using a near infrared probe 1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine

机译：使用近红外探针1,1'-二磺丁基-3,3,3'，3'-四甲基吲哚并花青分光光度法测定DNA

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris-HCl buffer, the reaction of DSTCY with DNA was complete within 10min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5-8.0 mu g ml(-1) and the detection limit of 45 ng ml(-1). (c) 2004 Published by Elsevier B.V.

机译：使用近红外探针1,1'-二磺丁基-3,3,3'，3'-四甲基吲哚基花青（DSTCY）开发了一种直接分光光度法测定溶液中DNA的方法。在pH = 7.5 Tris-HCl缓冲液中，在十六烷基三甲基溴化铵（CTAB）存在下，室温下10分钟内DSTCY与DNA的反应完成，这导致474 nm处的吸光度急剧增加。 DNA的浓度可以在这样的波长范围内确定，线性范围为0.5-8.0μg ml（-1），检测极限为45 ng ml（-1）。（c）2004年由Elsevier B.V.

著录项

来源
《Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy》 |2005年第9期|共5页
作者
Wang H; Li WR; Lu Y; Fu NN; Zhang HS;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
1; 1 '-disulfobutyl-3; 3; 3 '; 3 '-tetramethylindotrticarbocyanine (DSTCY); near infrared cyanine dyes; spectrophotometry; DNA; INDUCED FLUORESCENCE DETECTION; LIGHT-SCATTERING TECHNIQUE; RAPID COLORIMETRIC ASSAY; NUCLEIC-ACIDS; CAPILLARY-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; SERUM-ALBUMIN; STRANDED-DNA; DIODE-LASER; DYES;

机译：1;1'-二硫代丁基-3;3;3';3'-四甲基Insttrticarbocyanine（DSTCY）;近红外花青染料;分光光度法;DNA;诱导荧光检测;光散射技术;快速比色法;核糖酸;胶囊电泳;液相色谱;血清白蛋白;链状DNA;二极管激光器;染料;

相似文献

外文文献
中文文献
专利

1. Spectrophotometric determination of DNA using a near infrared probe 1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine [J] . Wang H, Li WR, Lu Y, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2005,第9期

机译：使用近红外探针1,1'-二磺丁基-3,3,3'，3'-四甲基吲哚并花青分光光度法测定DNA
2. Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5 '-dicarboxy-1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine [J] . Wang H, Li WR, Guo XF, Analytical and bioanalytical chemistry . 2007,第8期

机译：使用新型近红外花青染料分光光度法测定血清中的总蛋白5,5'-二羧基-1,1'-二磺丁基-3,3,3'，3'-四甲基吲哚并花青
3. Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine [J] . Hong Wang, Wei-Rong Li, Xiao-Feng Guo, Analytical and Bioanalytical Chemistry . 2007,第8期

机译：使用新型近红外花青染料5,5'-dicarboxy-1,1'-disulfobutyl-3,3,3'，3'-tetramethylindotricarbocyanine分光光度法测定血清中的总蛋白
4. Spectrophotometric Determination of Trace Hg~(2+) with hsDNA-modified Nanosilver Probe [C] . Xiaojing Liang, Guiqing Wen, Zhiliang Jiang International Conference on Chemical, Mechanical and Materials Engineering . 2013

机译：具有HSDNA改性的纳米单向探针的分光光度法测定痕量HG〜（2+）
5. Extending two-dimensional infrared spectroscopy to study DNA structure with native and non-native infrared probes. [D] . Krummel, Amber T. 2007

机译：扩展二维红外光谱，以利用天然和非天然红外探针研究DNA结构。
6. 3,3,3′,3′-Tetramethyl-6,6′-bis[(pyridin-4-yl)methoxy]-1,1′-spirobiindane monohydrate [O] . Ya-Jie Zhang, Yan Sun, Shu-Mei Gao, 2015

机译：3,3,3'，3'-四甲基-6,6'-双 [（吡啶-4-基）甲氧基] -1,1'-螺双茚满ane一水合物
7. Molecular Vibrations and Structures of High Polymers. V The Infrared Active Normal Vibrations of Isotactic Polypropylene, Poly(propylene-2-d), Poly(propylene-1,1-d2) and Poly(propylene-3,3,3-d3) in the 1500∼650 cm−1Region [O] . Tatsuo Miyazawa, Yoshiko Ideguchi 1963

机译：高聚物的分子振动和结构。 V在1500〜650cm-1region中，全同立构聚丙烯，聚（丙烯-2-d），聚（丙烯-1,1-d2）和聚（丙烯-3,3,3-d3）的红外主动正常振动
8. Initial Submission: Letter from Dupont Haskell Laboratory to USEPA Reporting Results in Acute Inhalation Study in Rats w/Propane, 1,1-Dichloro-1, 3,3,3-Tetrafluoro-, dated 09/15/1998 [R] . 1998

机译：初步提交：Dupont Haskell实验室致美国环保局报告的大鼠急性吸入研究报告，含有丙烷，1,1-二氯-1,3,3,3-四氟 - ，1998年9月15日

Spectrophotometric determination of DNA using a near infrared probe 1,1 '-disulfobutyl-3,3,3 ',3 '-tetramethylindotricarbocyanine

摘要

著录项

相似文献

相关主题

期刊订阅